摘要
目的建立反相高效液相色谱法同时测定龙胆花中落干酸、獐牙菜苦苷、龙胆苦苷、獐牙菜苷的含量。方法采用ZORBAX SB—CM18（250mm×4．6mm，5μm）色谱柱；流动相甲醇和水（含0．04％磷酸）为15：85的比例洗脱；检测波长为238nm：流速1ml·min^-1；柱温30℃。结果4种成分均达到基线分离，落干酸、獐牙菜苦苷、龙胆苦苷、獐牙菜苷的线性范围分别为0．10—6．25μg（r=0．9999），0．076—3．5μg（r=0．9998），0．04～5．65μg（r=0．9999）和1．27～7．62μg（r=0．9998）；平均回收率分别为99．2％（RSD=2．6％，n=5），103．0％（RSD=1．5％，n=5），101．0％（RSD=1．1％，n=5）．99．7％（RSD=3．6％，n=5）。结论该方法测定快速，结果准确、可靠。
Objective To establish a quantitative method of simultaneous determination of loganic acid, swertiamarin , gentiopicroside and sweroside in Gentiana veitchiorum Hemsl by RP - HPLC. Methods The sample was separated on the column of Zorbax S -C18 （250 mm ×4.6 mm, 5 μm） and eluted with methanol and water（0.04% phosphoric acid） to 15：85,the detection wavelength was at 238 nm, the flow rate was 1 ml· min^-1, and the column temperature was at 30℃. Results Four compounds were isolated. The linear ranges of loganic acid, swertiamarin, gentiopicroside and sweroside were 0.10 - 6.25 μg （ r = 0. 999 9 ） ,0. 076 3.5μg （r = 0. 999 8） ,0.04 - 5.65μg （ r = 0. 999 9） and i. 27 - 7.62 μg （r = 0. 999 8 ） respectively. The average recoveries were 99.2%（RSD=2.6%, n=5）,103.0%（RSD=1.5%,n=5）,101.0%（RSD=1.1%, n=5） and 99.7% （RSD= 3.6% ,n =5, ） ,respectively. Conclusion The method is rapid and precise .
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第8期1882-1883,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家教育部科学技术研究重点项目(No.205167)
青海省重点科技攻关项目(No.2006-N-141)
