摘要
为获得具有发育全能性的胚胎干细胞（ＥＳ细胞），对３日龄、３．５日龄和４日龄家兔胚胎的形态结构和免疫组化特征进行了观察。家兔胚胎的外围，不仅有透明带，还包有一层很厚的胶膜。３日龄兔胚为桑椹胚；３．５日龄胚胎已出现囊胚腔，囊胚腔较小，扩增内细胞团（ＩＣＭ）占据囊腔的１／２～２／３；４日龄为囊胚，囊腔扩大，ＩＣＭ与囊胚腔的体积比值缩小。桑椹胚的卵裂球和３．５日龄、４日龄胚胎的ＩＣＭ，以碱性磷酸酶染色和胚胎阶段特异性细胞表面抗原－１（ＳＳＥＡ－１）免疫荧光标记均呈强阳性，说明它们是由未分化的细胞构成。因此，从保证全能性的角度考虑，３日龄、３．５日龄和４日龄的家兔胚胎，均可用于ＥＳ细胞的分离。
Some morphological and immunohistochemical characteristics of the rabbit embryos collected 3,3.5 and 4 days post coitus (PC) were observed. The results showed that the rabbit embryos were surrounded not only by zona pellucida, but also by a thick mucin coat which might postpone the hatch of embryos in vitro. Embryos 3 days PC were morulae,embryos 3.5 days PC formed small blastocoels and the inner cell masses(ICMs) accounted for 1/2￣1/3 of the blastocoels. The blastocoels in embryos 4 days PC expanded dramatically and the proportion of volume between ICMs and blastocoel decreased.The blastomeres of morulae and ICMs of embryos 4 days PC revealed the positive reaction when stained with alkaline phosphatase(AKP)and stage specific embryo antigen 1(SSEA 1). This suggested that they were composed of undifferentiated cells and that the embryos of 3 , 3.5 and 4 day old could be used for isolation of ES cells with totipotency.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第4期351-354,共4页
Chinese Journal of Veterinary Science
基金
中国科学院院长基金
关键词
兔
胚胎干细胞
胚胎
生物学特征
rabbit
embryonic stem cell(ES)
embryo
biological characteristics
