摘要
采用ＰＣＲ扩增方法，从成熟猕猴桃果实组织中克隆到一个８２４ｂｐ的脂氧合酶（ＬＯＸ）基因片段（ｐＫＬＣ１），该片段含有２７５个氨基酸组成的开放阅读框架，它与拟南芥菜、黄瓜、豌豆、土豆、水稻、大豆、烟草和番茄的核苷酸同源性为５９．０％～７４．６％，氨基酸同源性为６３．１％～７６．３％。Ｓｏｕｔｈｅｒｎ杂交表明，ｐＫＬＣ１片段为一低拷贝数基因家族所编码。
An 824bp cDNA fragment(pKLC1)encoding part of the lipoxygenase(LOX)protein was cloned by PCR from ripening Actinidia chinensis fruit.pKLC1 has an open reading frame containing 275 amino acids. Comparisions between pKLC1 sequence and others from Arabidopsis ,cucumber,pea,potato,rice,soybean,tobacco and tomato indicated nucleic acid homology between 59% and 75%,and amino acid identity between 63% and 76%. Southern analysis confirmed that pKLC1 is encoded by a low copy number gene in A.chinensis.
出处
《园艺学报》
CAS
CSCD
北大核心
1998年第3期230-235,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金
浙江省自然科学基金
农业部"九五"重点项目资助
关键词
猕猴桃
果实
脂氧合酶
基因
克隆
Actinidia chinensis
Fruit
Lipoxygenase
Gene
