摘要
采用超声波提取法,通过单因素试验考察了乙醇浓度、提取时间、料液比、提取温度4个因素对留兰香总黄酮提取工艺的影响,并优选了正交试验L9(34)的超声提取最佳工艺条件。最佳工艺条件为:乙醇浓度50%,提取时间40 m in,料液比1∶50,提取温度60℃。其中乙醇浓度对黄酮提取影响最大,此工艺条件下提取的留兰香总黄酮含量为0.0951 mg/mg。其线性回归方程为A=11.51143C-0.00029,相关系数r=0.9999。该方法操作工艺简便,快捷,准确。
The optimal conditions of total flavonoids extraction from Mentha spicata were identified by orthogonal test. The results showed that the ethanol concentration and the ultrasonic time had significant effect on the extraction of total flavonoids and the optimum conditions of extraction were A3 B3 C1 D1 , which were as follows： the ethanol concentration was 50%, the ultrasonic time was 40 min, the solid-liquid ratio was 1 g： 50 mL and the temperature of extraction was 60℃, On the basis of the extracting method above, the content of total flavonoids in Mentha spicata was 0. 0951 mg/mg. The linear equation was A = 11. 51143C -0. 00029,r =0. 9999. This method is simple, quick and accurate with good prosperity.
出处
《中药材》
CAS
CSCD
北大核心
2009年第8期1288-1290,共3页
Journal of Chinese Medicinal Materials
关键词
留兰香
总黄酮
超声提取
Mentha spicata L.
Total flavonoids
Ultrasonic extraction
