摘要
以柑桔异源四倍体体细胞杂种[哈姆林甜橙（CitrussinensisOsbeckcv．Hamlinsweetorange）＋粗柠檬（C．jambhiriLush cv．Rough lemon）]为父本与单胚的二倍体柑桔类型[宜昌橙（C．ichangensisSwingle）与华农本地早（C.reticulataBlancocv．Huanongbendizao）的杂交后代]杂交，通过幼胚早期离体培养，获得了113株再生植株，其中89％为三倍体，并初步筛选出了适宜的幼胚离体培养时间为授粉后80d。
113 Plantlets were obtained from the cross between allotetraploid somatic hybrid [Hamlin (Citrus sinensis Osbeck) +Rough lemon (C. jambhiri Lush)] (as pollen parent )and mono-embryonic diploid citrus type[Ichang Papeda (C. ichangensis Swingle) × Huanong bendizao (C. reticulata Blanco)], 89% among them were triploid. It was found that the optimum period for young embryo culture in vitro was 80 days after pollination.
出处
《果树科学》
CSCD
北大核心
1998年第3期212-216,共5页
Journal of Fruit Science
基金
国家及湖北省自然科学基金
关键词
柑桔
体细胞杂种
三倍体
胚培养
Citrus
Somatic hybrid
Triploid
Embryo culture
