摘要
在建立‘藤稔’等５个葡萄品种离体培养快速繁殖体系的基础上，通过热处理和微茎尖脱毒培养获得了再生植株；并经过ＰＡＳ－ＥＬＩＳＡ和长臂光敏生物素标记ＧＦＬＶ－ＣＰ基因ｃＤＮＡ探针检测．结果表明白天３８℃夜晚３５℃处理脱除ＧＦＬＶ效果最佳；多次继代培养也有脱除ＧＦＬＶ的作用．从而首次获得了‘藤稔’、‘红地球’、‘９３０７’３个葡萄品种的脱除ＧＦＬＶ的试管苗．ＰＡＳ－ＥＬＩＳＡ与探针平行检测的结果还表明。
A rapid propagation in vitro system of 5 grapevine cultivars ‘Teng Ren’etc was established at first,then microapical plantlets were obtained through microapical culture and heat treatment, in which Grapevine Fan Leaf Virus (GFLV)was detected by PASELISA and GFLV coat protein(CP) gene cDNA probe labled by long arm photosensitive biotin. The results show that the heat treatment of 38℃(day) and 35℃(night) was more effective to release GFLV, longterm tissue culture of more than 20 generations have the effect of releasing GFLV.GFLVfree plantlets of ‘Teng Ren ’,‘Red Globe’, ‘9307’have been achieved for the first time. The paralleling detection of ELISA and probe indicated that it is reliable to take P/N<1 as standard of negative sample in PASELISA detection.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
1998年第4期54-58,共5页
Journal of Beijing Forestry University
关键词
葡萄
热处理
微茎尖培养
ELISA
探针检测
grapevine , heat treatment , microapical culture , GFLV detect, PASELISA, GFLVCP gene cDNA probe
