摘要
以徐薯１８、豫薯８号等品种为试材，对添加不同生长调节剂培养基的４个品种甘薯茎尖进行分生组织培养和脱毒苗快速繁殖研究。结果表明，甘薯茎尖采用体积分数为００５的次氯酸钠溶液消毒１０ｍｉｎ，切取０３～０５ｍｍ长的茎尖分生组织，在附加ＢＡ２２２μｍｏｌ·Ｌ－１，ＩＡＡ０５７μｍｏｌ·Ｌ－１和ＧＡ３０２９μｍｏｌ·Ｌ－１的ＭＳ培养基中，４个品种的茎尖培养成苗率为６８１％～８６６％，成苗期４６～６４ｄ转入第二培养基的时间以１８～２１ｄ较好，ＭＳ＋ＮＡＡ２６９μｍｏｌ·Ｌ－１培养基对甘薯无毒苗的快速繁殖更有利。以蛭石为移栽基质。
By applying cultivars Xushu 18 and Yushu No.8 et al as materials the factors affecting the tips meristem culture and the rapid propagation of virusfree seedling of sweet potato were studied.The results showed that four cultivars were cultured with 68.1%～86.6% plantlet ratio and 46～64 days plantlet period when the tips meristem of sweet potato(0.3～0.5mm long,disinfected 10 minutes by 0.05 sodium subchlorate solution)were cultured on the MS medium supplemented with 2.22μmol·L-1 BA,0.57μmol·L-1 IAA and 0.2μmol·L-1 GA3The suitable time for transplanting them into second medium was 18～21days.MS medium added 2.69μmol·L-1 NAA would be better for the rapid propagation of virusfree seedling of sweet potato.When vermiculite was used as the transplant medium,the survival rate of virusfree plantlet could reach 100%.
出处
《河南农业大学学报》
CAS
CSCD
1998年第2期133-137,共5页
Journal of Henan Agricultural University
基金
河南省科委重点科技攻关项目
