摘要
目的观察川芎嗪对支气管哮喘患者气道炎症的作用以及对转录因子T-bet和GATA-3表达的影响。方法60例哮喘患者随机分成常规治疗组(A组)和川芎嗪联合治疗组(B组),每组30例。另外选取健康人30例做为正常对照组(C组)。观察治疗前后的临床疗效,采用ELISA法检测干扰素γ(IFN-γ)和白介素4(IL-4)水平,采用RT-PCR法测定T-bet和GATA-3的表达。结果治疗前A、B组的T-bet、IFN-γ水平明显低于C组,GATA-3、IL-4水平明显高于C组(P<0.05),A、B组间没有显著差异;与治疗前比较,治疗后A、B组的T-bet、IFN-γ以及GATA-3、IL-4水平均有显著性差异(P<0.05);治疗后B组的T-bet和IFN-γ水平均高于A组,GATA-3和IL-4水平均低于A组(P<0.05)。结论在常规哮喘治疗的基础上,加用川芎嗪可以取得更好的疗效。川芎嗪通过纠正哮喘患者的免疫失衡来治疗哮喘,为川芎嗪对哮喘的治疗进一步提供了理论依据。
Objective To observe the effects of ligustrazine on airway inflammation and transcription factor T-bet and GATA-3 expression in elderly patients with asthma. Methods Sixty elderly patients with asthma were randomly divided into routine treatment group（group A） and ligustrazine group（group B）. Thirty healthy subjects were chosen as normal group （group C）. The concentrations of IL-4 and interferon-γ （IFN-γ）were measured by enzyme-linked immunosorbent assay（ELISA）. The expressions of T-bet and GATA-3 were measured by reverse transcription-polymerase chain reaction （RT-PCR）. Results Before treatment, the levels of T-bet and IFN-~ were lower and the levels of GATA-3 and IL-4 were higher in group A and B than those in group C. There were no significant differences in the levels of T-bet, IFN-γ, GATA-3 and IL-4 between group A and group B. After treatment, the levels of T-bet and IFN-γ were significantly increased and the levels of GATA-3 and IL-4 were significantly decreased. There were significant differences in the levels of T-bet, IFN-γ, GATA-3 and IL-4 between group A and group B after treatment. Conclusions The combination therapy of routine treatment and ligustrazine could get better efficacy in treating asthma.
出处
《实用老年医学》
CAS
2009年第4期264-266,共3页
Practical Geriatrics
基金
江苏省中医药局课题(200702)
