摘要
目的:观察鸡鸣散(槟榔、陈皮、木瓜、吴茱萸、紫苏、桔梗和生姜)抗凝血作用及对血液流变学的影响,探讨其昼夜节律性差异,验证前人关于鸡鸣散择时用药的合理性。方法:毛细玻管法观察小鼠凝血时间(CT)及其昼夜差异;Quick氏一期法观察大鼠的凝血酶原时间(PT)及其昼夜差异;采用皮下注射肾上腺素加冰水浴的方法复制血瘀证动物模型,观察鸡鸣散对"血瘀证"大鼠血液流变学的影响;Born氏比浊法观察大鼠血小板聚集。结果:鸡鸣散能明显延长CT、PT,且作用呈昼夜差异,夜间用药CT、PT延长显著优于白昼;模型组血液流变学各指标明显升高;与模型组比较,鸡鸣散能降低全血黏度、血浆黏度及纤维蛋白原黏度,夜间用药作用显著优于白昼;能显著抑制二磷酸腺苷(ADP)诱导的血小板聚集。结论:鸡鸣散具有抗凝血作用,能明显改善血瘀模型大鼠的血液流变学异常,夜间用药效果优于白昼。
AIM： To observe effects of Jiming Powder （Semen arecae, Pericarpium citri reticulatae, Fructus Chaenomelis , Fructus Evodiae , Folium Perillae , Radix Platycodonis , and Rhizoma Z ingiberis recens ） on anticoagulation and hemorheology and on the circadian variations in order to verify the rational practice of timing administering the drug by our ancestors. METHODS： The effect of Jiming Powder on clotting time（CT） was observed by using glass-tube method in mice. Quick test was used to determine the prothrombin time（PT） and circadian variations in rats. The experimental animal models of blood stasis syndrome were prepared by subcutaneous injection of adrenaline in combination with ice water bath for observing the effect of Jiming Powder on hemorheology in such types of animals, and the maximum rate of platelet aggregation was determined by method of Born turbidimetry. RESULTS： Jiming Powder could significantly delay CT and PT characteristic of circadian rhythm. As compared with the administration at daytime, CT and PT were greatly prolonged during the night. Besides, the hemorheological parameters were improved markedly in blood, plasma and fibrinogen viscosity, which suggested that the drug administration was more effective during the night than daytime. Also, the findings showed that Jiming Powder could prevent platelet aggregation induced by adenosine diphosphate. CONCLUSION： Jiming Powder plays a role in anticoagulation and improves the indication of hemorhelogy in the blood-stasis rats model, showing dosing timedependent.
出处
《中成药》
CAS
CSCD
北大核心
2009年第8期1171-1175,共5页
Chinese Traditional Patent Medicine
基金
安徽省高等学校青年教师自然科学科研资助项目(2007jq1174)
关键词
鸡鸣散
昼夜节律
抗凝
血液流变学
Jiming Powder
circadian rhythm
anticoagulation
hemorheology
