摘要
用重叠延伸PCR(overlap extension PCR)法获得轮状病毒VP4基因。根据Genbank中鼠VP4基因的序列设计34对引物,用overlap PCR法合成VP4全基因的两个片段A、B,将A、B分别连入pMD19-T simple载体,测序结果显示,成功合成了VP4全基因。证明了重叠延伸PCR法是获得目的基因的有效方法。
It was to synthesize the murine rotavirus VP4 antigen gene with overlapping PCR. According to the sequence of murine rotavirus antigen gene VP4,34 pairs of primers were designed. The full-length VP4 gene was synthesized through overlapping PCR and cloned into pMD19-T simple vector. Sequencing results showed that the production of PCR was correct. Overlap extension PCR was proved as a utility method to synthesize long-length gene.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第8期118-119,124,共3页
Biotechnology Bulletin
基金
国家自然科学基金项目(30671865)
