摘要
为研究高氟对雏鸡法氏囊的影响,选用1日龄AA肉鸡健雏300只,随机分为4组,分别饲以对照日粮(F23 mg.kg-1)和高氟日粮(F 400 mg.kg-1,高氟Ⅰ组;F 800 mg.kg-1,高氟Ⅱ组;F 1 200 mg.kg-1,高氟Ⅲ组)42d,以实验病理学和流式细胞术的方法观察雏鸡法氏囊的变化。结果显示高氟Ⅱ组和高氟Ⅲ组法氏囊绝对质量和脏器指数显著低于对照组(P<0.05或P<0.01),法氏囊淋巴细胞明显减少。经流式细胞仪测定,高氟Ⅰ组细胞周期与对照组相比差异不显著(P>0.05),高氟Ⅱ组和高氟Ⅲ组则出现明显变化。14日龄时高氟Ⅱ组和高氟Ⅲ组法氏囊淋巴细胞G0/G1期显著升高(P<0.05),G2+M期和增殖指数(PI)与对照组相比不同程度地降低;28日龄时高氟Ⅱ组和高氟Ⅲ组S期显著升高(P<0.01),凋亡率也显著升高(P<0.01);42日龄时高氟Ⅱ组和高氟Ⅲ组G0/G1期显著升高(P<0.01),S期、G2+M期和增殖指数(PI)显著降低(P<0.01),同时凋亡率显著高于对照组(P<0.01)。电镜观察,42日龄时高氟Ⅱ组和高氟Ⅲ组法氏囊可见多量的凋亡淋巴细胞及淋巴细胞线粒体肿胀。结果表明,日粮含氟达800及1 200 mg.k g-1时可引起法氏囊淋巴细胞增殖分化受阻,凋亡细胞显著增多,法氏囊生长发育受抑制,机体体液免疫功能受损。
The experiment was conducted to study the effects of high fluorine on the bursa of Fabricius in chickens by the methods of experimental pathology and flow cytometry （FCM）. 300 one day-old Avian broilers were divided into four groups and fed with Control diet（F 23 mg ·kg^-1） or High fluorine diets（F 400 mg ·kg^-1 , High fluorine group Ⅰ ; F 800 mg ·kg^-1 , High fluorine group Ⅱ ; F 1 200 mg ·kg^-1, High fluorine group Ⅲ ） for 6 weeks. The results showed that the weight and the relative weight of bursa of Fabricius were decreased（P〈0.01 or P〈0.05）, and lymphocytes were histopathologically decreased in number in high fluorine group Ⅰ1 and high fluphase and apoptosis-rates of high fluorine groups Ⅱ and Ⅲ were abnormally increased（P〈0.01） at 28 days of age. The G0/G1 phase was significantly increased（P〈0.01） and S phase,G2 ＋M phase and PI decreased（P〈0.01） in high fluorine groups Ⅱ and Ⅲ at 42 days of age. Also, the percentage of cellular apoptosis was higher in high fluorine groups Ⅱ and Ⅲ than in control group at 42 days of age. Typical morphological changes of apoptotic cells and some swelled mitochondria of the bursa of Fabricius in high fluorine group Ⅱ and high fluorine group Ⅲ were uhrastructurally observed. It is concluded that fluorine exceed 800 and 1 200 mg · kg^-1 in diet can cause differentiation and proliferation retardation of lymphocyte in the bursa of Fabricius and increased apoptotic cells, which leads to inhibition on the development of the bursa of Fabricius and impaired the function of humoral immunity in chickens.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第8期1235-1243,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省教育厅重点项目(2006A003)
四川省科技厅重点项目(07JY027-022)
关键词
高氟
法氏囊
病理变化
细胞周期
凋亡
雏鸡
high fluorine
bursa of Fabricius
lesion
cell cycle
apoptosis
chicken
