摘要
为了分析中国鸭肝炎病毒(DHV)的遗传变异和进化关系,作者测定了中国不同地区分离的7株DHV及1株疫苗株的全基因组序列,并与公布的40株DHV序列进行比较分析。结果发现VP1变异程度较大,高变区主要集中在180-194位和213-219位;不同毒株的潜在N-糖基化位点存在较大差异。多数基因的进化分析均表现出一致的结果,测序的8个毒株均分布在同一个遗传谱系,属基因A型,与基因B型和基因C型遗传距离较远,不在同一分支上。而JYZ02株与R85952株的遗传距离最近,属同一个亚支,提示JYZ02株可能由毒株R85952演化而来。结合氨基酸序列相似性分析和代表毒株的血清交叉中和试验结果,表明近年分离并测序的上述7个毒株未发生抗原变异。从多毒株进化分析结果可以看出,血清1型DHV仍是我国流行的优势血清型。
This experiment was conducted to study the genetic variations and phylogenetic analysis of Duck Hepatitis Virus（DHV） in China. The complete nucleotide sequences of 7 isolated strains and 1 vaccine strain of DHV-1 had been determined and comparative genome analysis with other DHV strains available in GenBank was performed. The results showed that the VP1 was the most variant gene and hypervariable regions were found in 180-194aa and 213-219aa of VP1 protein. Variant N-Linked glycosylation sites were found in different strains. Phylogenetic and evolutionary analysis showed that our sequencing strains belonged to genotype A, which were on different branches from genotype B and C. Strain JYZ02 had a very close relationship with strain R85952 which suggested that strain JYZ02 was evolved from strain R85952. The results of amino acid sequence identity and virus neutralization test proved that there was no antigenic variation occurred in the seven strains we determined. Phylogenetic analysis revealed that the dominant serotype of epidemic DHV in China was type 1 at present.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第8期1209-1214,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省自然基金项目(Z2006D06)
家畜疫病病原生物学国家重点实验室开放课题
关键词
鸭肝炎病毒
遗传变异
进化分析
duck hepatitis virus
genetic variations
phylogenetic analysis
