摘要
目的:探讨氯化三乙基锡(TETC)对体外培养大鼠C6胶质瘤细胞的增殖抑制作用及其机制,为胶质瘤治疗提供实验依据。方法:采用MTT法检测0.5、1.0和2.0μmol.L-1TETC对大鼠C6胶质瘤细胞作用24和48 h后细胞增殖抑制率,采用Hoechest33258染色荧光显微镜观察0.5、1.0和2.0μmol.L-1TETC作用48 h后C6胶质瘤细胞核的形态学变化,采用流式细胞术(FCM)检测0.5、1.0和2.0μmol.L-1TETC作用48 h后C6胶质瘤细胞周期和凋亡。结果:0.5、1.0和2.0μmol.L-1TETC在体外可抑制C6胶质瘤细胞增殖,24 h抑制率分别为7.92%、9.51%和19.03%;48 h抑制率分别为15.62%、36.16%和41.92%,存在剂量依赖性和时间依赖性上升趋势,48 h抑制率在各剂量组间及各剂量组与对照组间比较差异有显著性(P<0.05或P<0.01)。荧光显微镜显示TETC作用C6胶质瘤细胞48 h后,细胞呈现核浓缩和碎裂的凋亡形态学改变。1.0和2.0μmol.L-1TETC作用C6胶质瘤细胞48 h后,G0/G1期细胞比例明显高于对照组(P<0.05),S期细胞比例明显低于对照组(P<0.05),细胞凋亡比例明显高于对照组(P<0.05)。结论:TETC对大鼠C6胶质瘤细胞有增殖抑制作用,通过细胞周期阻滞及诱导凋亡可能是TETC抑制C6胶质瘤细胞增殖的作用机制。
Objective To study the inhibitory effect of triethyltin chloride（TETC） on proliferation of rat C6 glioma cells in vitro and its mechanism and provide basis for research on TETC in treatment for glioma.Methods MTT assay was performed to determine the inhibitory rate of 0.5,1.0 and 2.0 μmol·L^-1TETC on rat C6 glioma cells for 24 and 48 h.The changes of nucleus of C6 glioam cells treated with 0.5,1.0 and 2.0 μmol·L^-1TETC for 48 h were observed by fluorescent microscope.Flow cytometry was used to assess the effects of 0.5,1.0 and 2.0 μmol·L^-1TETC on cell cycle and apoptosis in rat C6 glioma cells for 48 h.Results 0.5,1.0 and 2.0 μmol·L^-1TETC inhibited the proliferation of C6 glioma cells in vitro,and the inhibitory rates were 7.92%,9.51%,19.03% and 15.62%,36.16%,41.92% in 24 h TETC treated group and 48 h TETC treated group,respectively.The inhibitory rate of TETC on C6 glioma cells determined by MTT assay increased in a dose-dependent and time-dependent manner,and there were significant differences of the inhibitory rates at 48 h between control and various doses groups as well as between various doses groups（P〈0.05 or P〈0.01）.Pyknotic nucleus and nuclear fragment were shown by fluorescent microscope.When C6 glioma cells were treated with 1.0 and 2.0 μmol·L^-1ETC for 48 h,the ratio of G0/G1 phase cells significantly increased compared with control group（P〈0.05）;while the cells at S phase were decreased（P〈0.05）;the apoptotic cells were significantly higher than those in control group（P〈0.05）.Conclusion TETC can inhibit the proliferation of rat C6 glioma cells in vitro,the mechanism of which may be involved in cell cycle arrest and apoptosis.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期595-598,F0002,共5页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30070645)
