摘要
用二倍体无核葡萄作母本与四倍体有核葡萄作父本进行杂交，用常规育种法去雄授粉，授粉后３５ｄ至５５ｄ期间，采用胚挽救技术，将各杂交组合的幼胚接种在不同培养基上进行离体培养。在设置的７个不同处理的杂交组合中，经系列培养后获５个杂交组合的胚培养苗１９株。经根尖染色体鉴定，有一杂交组合的两组试管苗为三倍体。经３年继代培养，目前已形成两个葡萄三倍体新品系。
Hybridization of grape breeding was conducted by crossing the diploid seedless grape as female parent with the tetraploid seedless grape as male parent.Based on the general breeding method of cutting the stamens with pollenization,the hybridized young embryo was inoculated on the different media using saving embryo techniques in the period of 35 to 55 days after pollenization.Seven treatments of crossing associations were set up and nineteen embryo breeding seedlings were obtained by raising five crossing associations systematically.According to the root tip chromosome appraisal,two groups of test tube seedings from one hybridized association were triploid.After three-year continuous breeding,the seedlings had developed into two new varities of grape triploid.
出处
《山东农业大学学报（自然科学版）》
CSCD
1998年第3期298-302,共5页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
育种
幼胚培养
根尖染色体
葡萄三倍体
general breeding
young embryo raising,root tip chromosome
grape triploid
