摘要
为提高抗克伦特罗(clenbuterol,CBL)单链抗体(single chian Fv,scFv)的表达量,考察了Mg2+、Ca2+、NH4+、NaHPO4-等无机离子,初始pH值以及装液量对重组抗体表达量的影响。优化试验结果表明,重组大肠杆菌E.coli BL21在初始pH值为7.2、含2 mmol/L Ca2+和20 mmol/L NaHPO4-的培养基中培养,装液量为25 mL,42℃诱导表达后抗CBL-scFv的表达量为33.7%。
The fermentation process of recombinant anti-clenbutero （CBL） single chain Fv （scFv） was studied in order to improve the expression level of recombinant protein. The effects of factors such as, concentration of inorganicions, i. e. Mg^2＋ , Ca^2＋, NH4^＋ and NaHPO4^-, pH ,and volume of medium were investigated. The results showed that the expression level of anticlenbuterol scFv was increased to about 33.7% of the total bacteria protein when recombinant E.coli was induced at 42℃ with pH 7.2, 25 mL volume of medium with 2 mmol/L Ca^2＋ and 20 mmmol/L NaHPO4^-.
出处
《广东农业科学》
CAS
CSCD
北大核心
2009年第8期200-202,共3页
Guangdong Agricultural Sciences
基金
国家自然科学基金(30871755&30400354)
国家"863"计划(2007AA10Z437)
广东省自然科学基金(06025825)
关键词
克伦特罗
重组抗体
发酵
clenbuterol
recombinant
fermentation
