摘要
目的探讨月见草花两种提取物对免疫抑制小鼠细胞免疫功能的影响。方法小鼠腹腔注射环磷酰胺(Cyclophosphamide,Cy)50mg/kg建立免疫抑制模型,然后将月见草花两种提取物分别以高、中、低三个剂量(低剂量437.5mg/kg、中剂量875mg/kg和高剂量1750mg/kg)每天1次灌胃,观察对小鼠脾脏T淋巴细胞增殖能力、肿瘤坏死因子-α(Tumor Necrosis Factorα,TNF-α)和干扰素-γ(Interferonγ,IFN-γ)分泌水平的影响。结果月见草花的两种中剂量提取物均可增加免疫抑制小鼠脾脏T淋巴细胞刀豆蛋白A(concanavalin A,ConA)刺激指数(SI值P<0.05),促进免疫抑制小鼠细胞因子TNF-α的分泌(P<0.05),中剂量的月见草花乙酸乙酯提取物还可以增加细胞因子IFN-γ的分泌水平(P<0.05);月见草花乙酸乙酯提取物对免疫抑制小鼠细胞免疫功能的促进作用显著强于水提取物(P<0.05)。结论月见草花乙酸乙酯提取物和水提取物均可明显增强环磷酰胺免疫抑制小鼠的细胞免疫功能,其中乙酸乙酯提取物的作用优于水提取物。
Objective To compare the effects of two different extracts of evening primrose flower on adaptive immunity in immunosuppressed mice. Methods The immunosuppressive mice models were induced by intraperitoneal injection of cyclophosphamide （50mg/kg）. T cell Proliferation response to ConA,the level of TNF-α and IFN-γ were determined after administering evening primrose flower extracts in high, medium and low-dose. Results The results showed that two different extracts of evening primrose flower at middle dose could cause a significant increase of the proliferation of splenocytees （ P 〈 0.05）, and the level of TNF-（ P 〈 0.05） in immunosuppressed mice, ethyl extract at middle dose also could cause an increase of IFN-（serum（ P 〈 0.05）;The effect of ethylacetate extract from evening primrose flower was significantly better than that of water extract （ P 〈 0.05）. Conclusion The extracts from evening primrose flower could significantly enhance the cellular immune function in immunosuppressed mice. Effects of ethylacetate extract was better than those of water extract.
出处
《宁夏医科大学学报》
2009年第4期430-431,437,共3页
Journal of Ningxia Medical University
基金
宁夏自然科学基金资助项目(编号:C1018)
关键词
月见草花
小鼠
细胞免疫
evening primrose flower
mice
adaptive immunity
