摘要
把WesternBlot分析抗原抗体反应的高度特异性与PVDF膜作为载体进行蛋白质氨基酸序列分析的高度敏感性相结合，对结核分枝杆菌的特异性抗原表位进行筛选和分析鉴定，获得了一个分子质量为31ku，N末端为AlaGluValAspTrpLeuValPheAlaValSerTrpProValGly的结核分枝杆菌抗原，与抗结核的单克隆抗体和结核病人血清发生阳性反应．特异性和保护性抗原的筛选为结核病的诊断、治疗及新型疫苗的构建打下了基础．
A specific 31 ku antigen of Mycobacterium tuberculosis was selected. and determined in combination of the high specificity of antigen-antibody reaction with the high sensitivity of amino acid sequence analysis on PVDF membrane. N-terminal sequence of the antigen is Ala Gin Val Asp Trp Leu Val Phe Ala Val Ser Trp Pro Val Gly. It has positive reaction with anti-M. tuberculosis MAb and with serum of TB patients. Selection of specific and protective antigens is the basis of tuberculosis diagnosis, theraphy and vaccine design.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期532-536,共5页
Journal of Fudan University：Natural Science
