摘要
对一个水稻珍汕97A不育系核DNA来源的具有自主复制功能的高度重复顺序片段ARS2（全长4270bp）进行了亚克隆构建和测序，获得了它的全顺序．该片段富含AT（69％），有2个ARS核心顺序（A／TTTTAPuTTTA／T），7个类核心顺序，其上下游20～50bp内有TNTPuAA的ARS特征顺序；并分析得出它的2个ARS功能关键区域（分别长400bp和360bp）．同时，还首次发现了具有较高多态性的水稻微卫星DNA（AT）n．
Subcloning and sequencing have been performed on a highly repetitive fragmentwith autonomously replicating capability from rice Zhenshan 97A nucleic DNA, whose lengthis 4 270 bp. The DNA segments were sequenced. It has high AT ratio (69%), two ARS core sequences(A/TTTTAPuTTTA/T) and 7 similar ones which comes with ARS characteristic sequence TNTPuAA at its upstream and/or downstream. Two key regions of ARS function are delimited in it with length of 400 bp and 360 bp. A microsatellite of rice locus genome(AT)n with high polymorphism was observed for the first time.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期469-476,共8页
Journal of Fudan University：Natural Science
关键词
高度重复序列
自主复制序列
水稻
微卫生DNA
highly repeated sequences
autonomously replicating sequences
microsatellite DNA
