摘要
WesternBlot实验证实了结核分枝杆菌（Mycobacteriumtuberculosis）H37Ra的单克隆抗体与自身抗原组蛋白发生免疫交叉反应．利用计算机软件对交叉反应的分子生物学基础进行了分析，发现结核分枝杆菌H37Ra的蛋白与组蛋白H1，H2A，H2B，H3，H4各有一个包含3个或4个氨基酸的序列同源区域．
Cross-reaction of Mycobacterium tuberculosis H37Ra monoclonal antibody (MAb)with self-antigen histone were demonstrated by western Blotting analysis. The molecular basis of the cross-reaction was investigated by amino acid sequence analysis using computer software. Homologous sequence of three or four amino acids between H37Ra protein and histone were found.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期419-422,共4页
Journal of Fudan University：Natural Science
基金
复旦大学遗传工程国家重点实验室资助
关键词
结核分枝杆菌
组蛋白
免疫交叉反应
单克隆抗体
Mycobacteria tuberculosis
histone
immune cross-reaction
molecular mimicry
