摘要
目的:研究丹参对内毒素脂多糖(lipopolysaccharide,LPS)激活后巨噬细胞高迁移率族蛋白B1(high mob-ility group box 1,HMGB1)释放和内毒素血症小鼠血清HMGB1水平的影响。方法:使用不同浓度的丹参提取液处理100 ng/ml LPS激活的培养巨噬细胞株RAW 264.7,观察培养上清液中HMGB1水平和细胞HMGB1 mRNA表达的变化,并通过腹腔内注射LPS建立的内毒素血症小鼠模型,观察丹参提取液对内毒素血症小鼠血清HMGB1水平和存活率的影响。细胞培养上清液和血清HMGB1含量采用酶联免疫分析方法检测,细胞HMGB1 mRNA表达采用RT-PCR方法检测。结果:丹参提取液明显抑制LPS激活后的巨噬细胞HMGB1释放和HMGB1 mRNA表达,降低内毒素血症小鼠血清HMGB1水平并提高存活率。结论:丹参有抑制巨噬细胞HMGB1释放和保护内毒素血症小鼠的作用。
Objective： To study the effects of Salvia miltiorrhiza on the release of high mobility group box 1 （ HMGB1 ） from endotoxin-stimulated macrophages and serum HMGB1 level of endotoxemia mice. Methods ： HMGB1 concentration in the culture medium and expression of HMGB1 mRNA were analysed by enzyme-linked immunosorbent assay and RT-PCR, respectively, after 100 ng/ml lipopolysaccharide（ LPS）- stimulated macrophage-like RAW 264.7 cells were treated with different doses of Salvia miltiorrhiza extract. In addition, the effect of Salvia miltiorrhiza extract was investigated on serum HMGB1 level and survival rate of endotoxemia mice which were established by intraperitoneal injection of LPS. Results： Salvia miltiorrhiza extract markedly suppressed the release of HMGB1 and expression of HMGBI mRNA in LPS-stimulated macrophages, and decreased serum HMGB1 level of endotoxemia mice with the survival rate elevated. Conclusion： Salvia miltiorrhiza suppressed extracellular release of HMGB1 in endotoxin-stimulated macrophages, and conferred protection against endotoxin lethality.
出处
《江苏大学学报（医学版）》
CAS
2009年第4期307-310,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏省自然科学基金资助项目(BK2006027)
江苏省中医药局科研资助项目(H05178)
