摘要
目的建立测定血浆中替米沙坦浓度的高效液相—荧光色谱分析方法。方法血浆样品以乙醚为提取溶剂处理,分析柱为KromsailKR100-5C18色谱柱（5μm,150 mm×4.6 mm）。流动相为0.01 mol/L KH2PO4水溶液（pH3.5）：乙腈=54：46（V/V）,激发波长Ex=305nm,发射波长Em=365 nm,流速1.0 ml/min,柱温为室温,进样量20μl。结果替米沙坦在5.0～500 ng/ml范围内线性良好（r=0.999 8）,最低检测浓度为2.5 ng/ml（S/N〉3）。该方法的相对回收率为（100.7±4.2）%（n=5）,绝对回收率为（88.4±3.9）%（n=5）,日内RSD为（3.1±1.0）%（n=5）,日间RSD为（4.0±1.5）%（n=5）。结论本方法准确、灵敏、特异性强、重显性好,适用于血浆中替米沙坦浓度测定和体内药代动力学研究。
Objective To establish a HPLC-FLU method for the determination of telmisartan concentration in human plasma.Methods The plasma examples were extracted with ethoxyethane.The separation was performed on Kromsail KR100-5C18（5μm,150 mm×4.6 mm）at room temperature with mobile phase consisted of 0.01 mol·L-1 KH2PO4 water solution（pH 3.5）;cyanomethane=54：46（V/V）.The λEx was set at 305nm and the λEm was at 365nm,and the sample size was 20μl.Results The linear range of telmisartan was 5.0500ng·ml-1（r=0.999 8）,with the lowest detectable limit at 2.5ng·ml-1（S/N〉3）.The relative recovery rate was（100.7±4.2）%（n=5）and the absolute was（88.4±3.9）%（n=5）.The intra-day RSD was（3.1±1.0）%（n=5）and the inter-day was（4.0±1.5）%（n=5）.Conclusion The method is simple,sensitive,accurate and reproducible,and suitable for the determination and pharmacokinetic study of telmisartan in human plasma.
出处
《临床军医杂志》
CAS
2009年第4期706-707,共2页
Clinical Journal of Medical Officers
