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水稻端粒相关序列的克隆及鉴定 被引量:1

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摘要 端粒不仅包括简单的端粒重复序列 ,也包括较复杂的端粒相关序列 .参照拟南芥菜的端粒重复序列 (TTTAGGG) n,设计了 2个引物 :(TTTAGGG) 3 和 (CCC TAAA) 3 CCC .利用单引物采取 2种方法在水稻中进行PCR圹增 ,得到了 8个片段 .然后用Bal31酶解和RFLP图谱定位的方法检测了它们的亚端粒特性 .用此方法鉴定了 6个端粒相关序列 ,分别定位于水稻第 5 ,6 ,7,9号染色体 . 1 RichardsEJ,AusubelFM .IsolationofahighereukaryotictelomerefromArabidopsisthaliana.Cell,1988,5 3:12 7~ 1362 CohnM ,EdstromJE .ChromosomeendsinChironomuspallidivittatuscontaindifferentsubfamiliesoftelomere associatedse quences .Chromosoma ,1992 ,10 1:6 34~ 6 403 WuTY ,WangYX ,WuR .TranscribedrepetitiveDNAsequencesintelomericregionsofrice (Oryzasativa) .PlantMolec ularBiology ,1994,2 6 :36 3~ 3754 GanalMW ,LapitanNL ,TanksleySD .Macrostructureofthetomatotelomeres.ThePlantCell,1991(3) :87~ 945 陈受宜 ,朱立煌 ,洪 建 ,等 ,水稻抗盐突变体的分子生物学鉴定 .植物学报 ,1991,3:5 6 9~ 5 73
出处 《中国科学(C辑)》 CSCD 1998年第4期324-332,共9页 Science in China(Series C)
基金 国家水稻基因组计划资助项目
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参考文献4

  • 1Slijepcevic D,Chromosoma,1996年,104卷,596页
  • 2洪德军,科学通报,1995年,40卷,13期,1229页
  • 3Wu T Y,Plant Mol Biol,1994年,26卷,363页
  • 4陈受宜,植物学报,1991年,3卷,569页

同被引文献18

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  • 5Wu T Y,Wang Y X,Wu R.Transcribed repetitive DNA sequences in telomeric regions of rice (Oryza sativa)[J].Plant Molecular Biology,1994,26:363-375.
  • 6Fajkus J,Kovarik A,Kralovics R,et al.Organization of telomeric and subtelomeric chromatin in the higher plant Nicotiana tabacum[J].Molecular and General Genetics,1995,247:633-638.
  • 7Zakian V A.Telomeres:beginning to understand the end[J].Science,1995,270:1601-1607.
  • 8Mao L,Devos K M,Zhu L,et al.Cloning and genetic mapping of wheat-associated sequences[J].Molecular and General Genetics,1997,254:584-591.
  • 9Singh R J,Hymowitz T.The genomic relationship between Glycine max(L.)Merr and G.soja Sieb and Zucc as revealed by pachytene chromosome analysis[J].Theoretical and Applied Genetics,1988,76:705-711.
  • 10Sykorová E,Lim K Y,Kunická Z,et al.Telomere variability in the monocotyledonous plant order Asparagales[J].Proceedings of Royal Society B Biological Science,2003,270:1893-1904.

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