摘要
目的探讨中药诱导小鼠肺组织β-防御素2、3的表达。方法将60只C57小鼠随机分为试验组和对照组,试验组分为4个药物组和2个对照组,每个药物组分为2个剂量组,分别腹腔注射小剂量和2倍剂量的黄芪、鱼腥草、板蓝根和香菇多糖,阴性对照组小鼠腹腔注射生理盐水,阳性对照组小鼠腹腔注射脂多糖。观察24h后处死,采取RT-PCR方法检测小鼠肺组织β-防御素2、3的表达。结果黄芪小剂量组和2倍剂量组检测到小鼠肺组织β-防御素2表达,两组间差异有统计学意义(P<0.01),黄芪2倍剂量组检测到β-防御素3表达。结论中药黄芪可诱导小鼠肺组织β-防御素2、3表达。
Objective To research wither the Chinese medicines can induce the expression of beta-defensin2 and 3 in pulmonary tissue and whither this reaction depend on the dose. Then find the effective induce products. Methods Sixty C57 mice were divided into four drug experimental groups and two control groups randomly, each drug experimental groups has twelve mice and each control group has six mice. And then divided each drug groups into two dose-groups. In experimental groups, the mice were injected Chinese medicines intraperiton. The mice in negative group were injected one milliliter normal saline and the positive group mice were injected LPS into intraperiton. After twenty-four hours ,lung tissue were harvested under sterile condition. Total RNA were extracted from pulmonary tissue and mBD-2,3 were measured by RT-PCR. Beta-aetin was used as internal stand- ard. DNA sequence were used to confirm the specificity of mBD-2,3 eDNA fragment. Results β-defensin-2 genes were detected in radix astragali group. And the level of expression increase more in the double dose group than half dose group（P〈0. 01 ）.β- defensin-3 genes were detected in double dose radix astragali group. Conclusion Chinese medicine Radix astragali can upregu- late β-defensin-2 and 3 genes expression in mouse pulmonary.
出处
《四川医学》
CAS
2009年第8期1208-1210,共3页
Sichuan Medical Journal
