摘要
目的：探讨茶多酚（ｔｅａｐｏｌｙｐｈｅｎｏｌｓ，ＴＰ）减轻阿霉素心脏毒性的作用及其机制。方法：以病理记分法记录心肌损害程度；用ＴＢＡ法测定大鼠心肌组织的脂质过氧化水平；用邻苯三酚自氧化法测定心肌组织、红细胞的ＳＯＤ活力；用ＤＴＮＢ法测定心肌ＧＳＨＰｘ活力。结果：阿霉素两周累积用量１６ｍｇ·ｋｇ－１（第２天，第４天１ｍｇ·ｋｇ－１；第６天，第８天２ｍｇ·ｋｇ－１；第１０天，第１２天３ｍｇ·ｋｇ－１；第１４天４ｍｇ·ｋｇ－１；ｉｐ）可致大鼠心肌损害，心肌ＭＤＡ水平升高，ＧＳＨＰｘ活性下降，心肌和红细胞ＳＯＤ活性降低，ＴＰ２８，５６和８４ｍｇ·ｋｇ－１具有减轻阿霉素所致的大鼠心肌毒性作用。结论：茶多酚减轻阿霉素心肌毒性机制可能与其清除自由基作用、保护心肌ＳＯＤ及ＧＳＨＰｘ活性有关。
OBJECTIVE: To investigate the effect of tea polyphenols (TP) and β carotene on reducing doxorubicin induced cardiotoxicity in rats. METHODS: The degree of myocardial damage was graded by the pathological score. The myocardial MDA value of rat heart was measured by TBA method. The pyrogallol autoxidation method was used for determination of SOD activity of heart and erythrocytes. The GSH Px activity of heart was assayed with DTNB method. RESULTS: Doxorubicin (Dox) of 16 mg·kg -1 was given ip to the rats intermittently (Dox 1 mg·kg -1 injected ip at day 2, day 4; day 6, day 8, 2 mg·kg -1 ; day 10, day 12, 3 mg·kg -1 ; day 14, 4 mg·kg -1 ) induced severe myocardial damage, increased myocardial malondialdehyde (MAD) content, decreased myocardial superoxide dimutase (SOD) and glutathione peroxidase (GSH Px) activities in rats. Administration of tea polyphenols (TP) 28, 56 or 84 mg·kg -1 ig per day reduced the cardiotoxicity induced by Dox. CONCLUSION: The mechanisms of reduced cardiotoxicity induced by Dox may depend on the effect of tea polyphenols on scavenging free radicals in rat heart, protecting the activity of SOD and GSH Px, and inhibiting lipid peroxidation.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
1998年第7期405-409,共5页
Chinese Pharmaceutical Journal
关键词
阿霉素
花多酚
心脏毒性
doxorubicin, tea polyphenols, β carotene, lipid peroxidation, superoxidedismutase, glutathione peroxidase
