摘要
目的通过对从污染地区分离出的抗汞菌株——溶解肠杆菌YH16的研究,获得新的抗汞操纵子基因,为汞污染环境的微生物修复打下基础。方法通过质粒消除实验确定抗汞基因的位置,通过构建基因文库的方式筛选获得新的抗汞操纵子基因(mer)。结果YH16的抗汞操纵子位于质粒上,该操纵子长达6048bp,由7个ORF组成,依次为merT、merP、merC、merA、merD、tnpA、tnpA,与典型的Tn501mer操纵子和Tn21mer操纵子相比,缺少merR。结论获得了缺少merR基因的mer操纵子,具有理论研究意义,并为抗汞工程菌的构建打下基础。
Objective In order to obtain the Hg-resistance operon, the gene library of mercury-resistance strain： Enterobacter dissolvens sp. YH16 was constructed. Methods Plasmid-knockout experiment had been used to posit the Hg-resistance gene, the gene library and bioinformatics analyzing tools had been used to screen the Hg-resistance gene. Results The mer genes were posited in the plasmid and organized as merT, merP, merC, merA, merD, tnpA, tnpA, closely related to that located in Tn501 and Tn21, but lacking merR. Conclusion A different mer operon gene was obtained, which might lay a basis for the further study on the structure analysis and gene clone of it.
出处
《广东药学院学报》
CAS
2009年第4期401-404,共4页
Academic Journal of Guangdong College of Pharmacy
关键词
抗汞质粒
质粒敲除
mer操纵子
汞污染
Hg-resistance plasmid
plasmid-knockout
mer operon
Hg pollution
