摘要
目的：研究中药参松养心胶囊对冠状动脉结扎大鼠心肌重构和室颤发生的药物作用特点。方法：雄性SD大鼠23只分为假手术组5只，模型组、胺碘酮组和参松养心胶囊组各6只。各组动物均于造模后第2天开始灌药，连续4周。4周后经飞利浦HDI5000超声诊断仪、15MHZ高频线阵探头，行心脏二维超声和多普勒检测各组大鼠心脏结构、心功能，并进行开胸后在体心脏电刺激检测室颤阈值及其持续时间实验。结果：经4周给药后，超声检测参松养心胶囊组室间隔舒张末期厚度（IVSTd）及左室舒张末期内径（LVDd）与模型组比较有显著性差异：（1．20±0．49）vs（0．78±0．08）mm和（6．77±1．34）vs（7．95±0．92）mm，（P值分别〈0．01和0．05）；胺碘酮组各项超声结果与模型组比较均无统计学差异（P〉0．05）。参松养心胶囊组实测左室心肌质量指数（LVMI）较模型组比较有统计学差异：（17．12±1．91）vs（18．95±1．41）g·m^-2（P〈0．05），而胺碘酮组则无统计学差异（P〉0．05）。对各组电生理检测室颤阈值及1～5，6～10，11～15V3阶段电刺激诱发室颤持续时间各组间有非常显著的差异（P〈0．01），16～20V阶段电刺激诱发的室颤持续时间各组间也有显著性差异（P〈0．05）。样本“均秩”胺碘酮组和参松养心胶囊组室颤阈值是模型组的4倍，室颤持续时间以胺碘酮组抑制效果最为明显。结论：冠状动脉结扎大鼠心脏是由容量负荷致心脏左室离心性肥大的模型，心室重构的结果影响心脏的电生理功能。胺碘酮提高室颤阈值的作用与其作用于心肌细胞膜的多种离子通道有关；参松养心胶囊也提高了室颤阈值，其作用机制除与该药作用于心肌细胞膜的多种离子通道外，还可能由于其抑制心室重构而改善其电生理的基质有关。
Objective： To study the effect of shensongyangxin capsule on myocardial remodeling and ventricular fibrillation characteristics in rat with coronary artery ligation. Method： Twenty-three male rats were randomly divided into sham-group （n = 5 ）, model group （n = 6 ） , anmiodarone group （n = 6 ） and shensongyangxin capsule group（ n = 6 ）. Drugs were administrated after modeling of 2 days, lasting four weeks. Two dimensional and Doppler images were acquired by a 15 MHz high-frequency linear ultrasound transducer at 4 weeks after operation, and chest was opened to detect ventricular fibrillation threshold value and persistent time. Result： After administration of four weeks, echocardiogram was detected. Compared with model group, shensongyangxin capsule group diastasis interventrlcular septum thickness （ IVSTd ） and left ventricle diameter （ LVDd ） were signifieiently different between them （ 1.20± 0.49） vs （0. 78 ±0. 08） mm and （6. 77 ± 1.34） vs （7. 95 ±0. 92） mm, （P 〈0. 01 and 0. 05） ; eehocardiogram result had no difference in amiodarone and model groups （ P 〉 0.05 ）. LBMI measured by practieion was different between shensongyangxin capsule and model groups： （17. 12 ± 1.91 ） vs （18. 95 ± 1.41 ） g · m^-2, （p 〈 0. 05）, while amiodarone group had no difference compared with model group. Electrophysiology was used to detect ventricular fibrillation threshold value and 1-5, 6-10, 11-15 V three stages＇ventricular fibrillation threshold persistent time were significiently different among each group （P 〈 0.01 ）, 16-20 V stage V ventricular fibrillation persistent time were also different among each group （ P 〈 0. 05 ）. Sample ＂average ranks＂ showed ventricular fibrillation threshold value of amiodarone group and shensongyangxin capsule group were four times than model group ; and amiodaron group had best effect of holding-back ventricular fibrillation persistent time. Conclusion： The coronary artery ligation can result in myocardial remodeling by increasing volume load, and at the same time influencing electrophysiology function of heart. Amiodaron elevated ventricular fibrillation threshold of heart, this effect maybe relate to influencing many ion channels of myocardial cellular membrane ; shensongyangxin capsule also elevate ventricular fibrillation threshold of heart, this effect maybe also relate to influencing many ion channels of myocardial cellular membrane, and on the other hand this effect maybe relate to hold-back ventricular remodeling after coronary artery was ligated, accordingly improve electrophysiological base material of heart.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第16期2101-2104,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30672754)
关键词
冠状动脉结扎
室颤阈值
心室重构
超声心动图
coronary artery ligation
ventricular fibrillation threshold value
ventricular remodeling
echocardiogram
