摘要
目的使用poly(I:C)模拟RNA病毒感染,建立一个安全有效的病毒感染模型,探讨病毒诱发气道的炎症。方法BALB/c小鼠随机分为对照组和poly(I:C)组,poly(I:C)组连续3d应用poly(I:C)滴鼻后,连续5d观测小鼠肺泡灌洗液(BALF)细胞学改变,ELISA测定IFN-γ浓度,同时观察小鼠肺病理改变。结果对照组小鼠BALF中中性粒细胞数和IFN-γ浓度分别为(1.53±0.06)×106/L和(7.11±1.11)ng/L。自Poly(I:C)滴鼻后第1天起,小鼠BALF中性粒细胞数和IFN-γ浓度开始升高,分别为(2.00±0.12)×106/L和(8.01±1.79)ng/L,肺间质内出现炎症细胞。这些变化在滴鼻后的第3天达到高峰,BALF中性粒细胞数和IFN-γ浓度分别为(4.30±0.32)×106/L和(13.51±1.40)ng/L,大量炎症细胞聚集在肺间质,随后炎症变化开始下降。对照组和poly(I:C)刺激组的BALF中淋巴细胞数均为(2.10±0.11)×106/L。结论Poly(I:C)可以模拟RNA病毒感染,诱发病毒感染相似的气道炎症,促使中性粒细胞聚集,破坏正常气道,刺激IFN-γ分泌。但是poly(I:C)诱发的气道炎症是有时间限制的。
Objective Respiratory viral infection is considered as the leading cause of respiratory disorders in children, including asthma, wheezing exacerbation, or secondary bacterial pneumonia. Most of respiratory viruses are single-strand RNA viruses, which produce double-strand RNA during their replicative cycles, so synthetic double-stranded RNA poly （I ： C） is usually used to mimic viral infection in vivo. To establish safe and effective animal model of real respiratory viral infection, poly （I ： C） was used to mimic real RNA infection in this research and its effects on airway inflammation was evaluated. Methods BALB/c mice were randomly divided into two groups： the control group and the poly （I ： C） group. The poly （I ： C） group was treated with poly （I ： C） for three days, and during the five days followed, the bronehoalveolar lavage fluid （BALF） and lung tissue of mice of the two groups were collected. Mice＇s airway inflammation was evaluated by accounting different types of cells in BALF, measuring the concentration of Thl type cytokine IFN-γ in BALF and investigating the pathologic severity. Results The number of neutrophils and concentration of IFN-γ in control＇s BALF were （1.53±0.06）×10^6/L and （7.11±1.11） ng/L respectively. Comparing with the control group, treating with poly （I ： C） would induce the increment of the number of neutrophils in BALF and the concentration of poly （I ： C） in BALF. These changes started the first day after treating with poly （I ： C）, （2.00±0.12）×10^6/L and （8.01±1.79） ng/L respectively. At the same time, inflammatory cells were recruited into lung tissue, including neutrophil . All of these changes were most remarkable on the third day, when the number of neutrophils and concentration of IFN-γ in BALF were （4.30±0.32）×10^6 /L and （ 13.51±1.40） ng/L respectively. After the third day the inflammatory changes began to decrease. However, both the control and poly （I ： C ） treating group had similar number of lymphocytes in BALF, （2.10±0.11）×10^6/L. Conclusions Poly （I ： C） could induce viral infection like airway inflammation, including neutrophils recruiting, enhancing IFN-y secretion, and pathological changes. However, the effects had time limitation.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2009年第8期768-771,共4页
Journal of Clinical Pediatrics
