摘要
样品经乙腈提取,碱性SPE小柱净化,采用Agilent C18柱,以乙腈∶0·017mol/L磷酸=1∶4为流动相洗脱,柱温30℃,流速1·0mL/min。用二极管阵列检测器DAD或紫外检测器270nm处检测,以保留时间定性,面积外标法定量。最低检出限0·005～0·01μg/mL,线性范围0·005～1·0μg/mL。在加标量分别为0·02、0·04mg/kg的水平上回收率均在80·78%～120·72%。本方法灵敏度高,能简便、快速、准确地测定畜禽肉中的磺胺类药物(SAs)残留。
After being extracted by acetonitrile, solid phase extration（ SPE）, eight sulphonamides residues in animal issues could be separated on Agilent C18 column using acetonitrile：0.017mol/L H3PO4 = 1：4 as the mobile phase at a flow rate of 1.0mL/min, and then simultaneously detected at 270nm, the column temperature was at 30℃.The quality was determined with retention time and the concentrations were determined with area calibrated curves.A good linear relationship exists for all residues in concentration range of 0.005-1.01μg/mL, while the detection limit were 0.005 to 0.01μg/mL.The recovery rates of sample were in the range of 80.78%-120.72% at the add level of 0.02 and 0.04mg/kg.Eight sulphonamides residues could be separated completely, which proved to be accurate and reliable.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第8期316-318,共3页
Science and Technology of Food Industry
基金
国家标准计划项目(20020303-T-424)
