摘要
目的：建立高灵敏度、快速、简便的测定高脂固体食品中甜蜜素含量的检测方法。方法：采用超声提取、沉淀去蛋白和脂肪、净化等手段对样品进行前处理,用毛细管气相色谱法测定。结果：甜蜜素含量在0.0050-0.20 mg/ml内线性关系良好,Y=9.277×10^5X＋2.971×10^3,r=0.9993。当取10 g样品测定时,测定范围为0.050-2.0 g/kg,最低检出限为0.014 g/kg。回收率在91.4%-95.1%之间,RSD为2.2%-5.7%。结论：本法灵敏度高、检测限低、线性范围宽,操作比较简便、快速,结果准确,适合于高脂固体食品和普通固体食品中甜蜜素含量的检测。
Objective： To set up a new method to test the content of sodium cyclamate in fat-rich solid food which is highly sensitive,swift,and convenient.Methods：Treat the sample with ultrasonic extraction,remove protein and fat by precipitation,and purification before testing by capillary gas chromatography.Results：Linear relation is fine with sodium cyclamate contents between 0.0050 and 0.20 mg/ml,Y=9.277×10^5X＋2.971×10^3,r=0.9993.When testing a sample of 10 grams,it ranges from 0.050 to 2.0 g/kg,with a lowest detection limit of 0.014 g/kg.Recovery ratio ranges from 91.4% to 95.1%,RSD from 2.2% to 5.7%.Conclusion：This approach enjoys advantages of high sensitivity,low detection limit,broad range of linear relation,comparatively more convenient to operate,time-saving,and accuracy.So it is suitable to test the content of sodium cyclamate in both fat-rich and normal solid food.
出处
《中国卫生检验杂志》
CAS
2009年第8期1809-1811,共3页
Chinese Journal of Health Laboratory Technology
关键词
高脂固体食品
甜蜜素
毛细管柱
气相色谱法
Fat-rich solid food
Sodium cyclamate
Capillary column
Gas phase chromatography
