摘要
目的：采用梯度淋洗离子色谱法快速测定蔬菜中吲哚乙酸含量。方法：选用IonpacAS11阴离子分析柱,0-20 mmol/L KOH淋洗液,流速为1.0 ml/min,电导检测器。用纯水萃取样品,经高速离心、过C18小柱、用0.25μm微孔滤膜过滤后直接进样。结果：本法相关性好（r=0.9990）,精密度高（RSD%〈5.0）,样品加标平均回收率为88.3%-101%,最低检出量0.10μg/g。结论：该法操作简单、快速、准确、灵敏,适用于大批样分析。
Objective： An ICS method was applied to simultaneously determine indol-3-acetic acid in vedetable.Methods：The method was based on ICS separation under the condition of isocratic eluent with 020 mmol/L KOH and flow rate of 1.0 ml/min by using of the IonPAC AS11 analytical column and suppressed conductivity detector.Results：It was showed that this method has good linear relativity（r=0.9990） and good precision（RSD%〈5.0）.The samples′average recoveries were 88.3%～101% and the detection limit were 0.10 μg/g.Conclusion：The method is simple,fast and of high sensitivity and precise results.This method can be applied widely and suitable for trace analysis of Indol-3-acetic acid in vegetables.
出处
《中国卫生检验杂志》
CAS
2009年第8期1796-1797,共2页
Chinese Journal of Health Laboratory Technology
基金
宁波市重点实验室建设基金(2008A22002)
关键词
吲哚乙酸
离子色谱
蔬菜
Ion chromatography
Vegetable
Indol-3-acetic acid
