摘要
鉴于纤维素酶研究和生产中，纤维素酶活力测定和酶活力单位的表示方法很不统一的现象，本文研究和分析了３，５－二硝基水杨酸法测定羧甲基纤维素酶糖化力的实验条件，如：温度、ｐＨ、酶促反应时间、ＤＮＳ显色时间、波长等．实验表明：在５０℃，ｐＨ４．６条件下酶促反应时间５ｍｉｎ，ＤＮＳ显色５ｍｉｎ，于４９０ｎｍ处测定ＯＤ值比较适宜．
In the studying and producing of cellulase ,the method of cellulase activity measurement and enzyme activity expression is very diverse .So in this paper ,the conditions for carboxy-methy cellose(CMC)enzyme activity measurement was studied with 3,5-dinitrosalicylic acid(DNS)method ,The results show as follows :the appropriate enzyme reaction conditions are pH4.6,50℃ and 5 miniutes,the proper color-reaction is 5 miniutes and the optimal absorb wavelength is at 490nm.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
1998年第3期66-69,共4页
Journal of Henan Normal University(Natural Science Edition)
关键词
纤维素酶活力
DNS法
羧甲基纤维素
cellulase activity
3,5-dinotrosalicylic acid
carboxymethylcellulose
