摘要
目的探讨联合应用利血平和1-甲基-4-苯基-1,2,3,6四氢吡啶(1-methyl-4-phenyl-tetrahydropyridine,MPTP)制备帕金森病小鼠模型的方法。方法采用单独应用利血平、MPTP及联合利血平和MPTP分别造成C57BL小鼠的帕金森病模型,对照组用同体积的生理盐水,测定各组小鼠爬杆时间检测动物运动协调性,应用免疫组织化学方法观察囊泡单胺转运体(VMAT2)和酪胺酸羟化酶(TH)在黑质致密部分布的变化。结果所有帕金森病模型小鼠都出现不同程度爬杆时间延长,免疫组织化学分析显示,帕金森病组小鼠较对照组小鼠VMAT2和TH阳性神经元细胞数目在黑质致密部明显减少。联合利血平和MPTP制备的C57BL小鼠帕金森病模型较利血平或MPTP诱导模型在病理和行为变化更为明显。结论联合应用利血平和MPTP是一种有效的制备帕金森病小鼠模型的方法。
Objective To investigate the feasibility of reserpine plus 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP） to induce Parkinson disease（ PD） mouse models. Methods C57BL mice were given reserpine, MPTP, or reserpine ＋ MPTP to induced PD model,respectively. The control mice were treated with the same amount of 0.9% saline. The pole test was conducted to determine the motor harmony,and the distribution of VMAT2 and TH in substantia nigra pars compacta（SNC） was examined by immunohistoehemical staining. Results All PD model mice showed a different degree of disability in pole test. The numbers of VMAT2-positive neurons and TH-positive neurons in SNC were significantly reduced in PD models than in the controls. Changes of pathology and behavior features in PD models by reserpine plus MPTP were more obviously than that by reserpine or MPTP. Conclusion Reserpine plus MPTP may be an effective way to induce C57BL mouse model of PD.
出处
《山西医科大学学报》
CAS
2009年第8期693-696,767,共5页
Journal of Shanxi Medical University
