摘要
建立了离子色谱-电导检测器直接进样测定苏氨酸的方法。淋洗液为4．0mmol／L的酒石酸和1．0mmol／L的2,6-吡啶二羧酸，流速为1．0mL／min，检测器为电导检测器。结果表明，苏氨酸在10～100mg／L的线性范围内线性良好，回归方程为：A=0．3649C-0．3315，R^2=0．9998，精密度实验RSD=1．393％，最小检测限为1．8mg／L；苏氨酸与其他阳离子同时测定时互不干扰；非离子态物质对苏氨酸的测定没有影响。离子色谱-电导检测器测定方法简单、准确，可用于苏氨酸的定量分析。
A new method was established for determination of threonine（Thr）. Ion chromatography with conductivity detector was adopted with tartaric acid（4.0 mmol/L） and 2,6- Pyridinedicarboxylate（1.0 mmol/L） as the eluent.the flow rate was 1.0 mL/min. The detection was performed by a conductivity detection mode. The regression equation with Thr was A=0.3649C-0.3315. The calibnation curves were linear with a range of 10- 100 mg/L, R2=0.9998. Precision test RSD was 1.393%. The coinstantaneous determination of Thr and other cations were achieved; non-iron component has no influence with the determination of threonine. This method is simple, accurate and suitable for the determination of Thr.
出处
《食品科技》
CAS
北大核心
2009年第8期280-283,共4页
Food Science and Technology
关键词
离子色谱
电导检测器
苏氨酸
ion chromatography（IC）
conductivity detector
threonine（Thr）
