摘要
目的对北京市2007年5—7月间发生并收集到的8起手足口病暴发疫情进行病原学分析及鉴定。方法采用RT-PCR技术，对手足口病患儿的疱疹液或咽拭子标本，直接进行肠道病毒（EV）分型；采用RD传代细胞，对标本进行病毒分离培养，再对其阳性培养物进行EV分型。对其中部分原始标本和阳性分离物中的EV VP1区进行核苷酸序列及预测的氨基酸序列分析。结果北京市2007年发生在大兴区的2起疫情由EV71引起，其他4个区的6起疫情均由柯萨奇病毒A16型（Cox A16）引起。大兴区2起疫情的EV71都属于C基因型的C4亚型，但分别处于VP1基因亲缘性系统发生树的不同分支，其核苷酸和氨基酸分别存在3．7％和0．8％的差异；来源于顺义区的CoxA16与其他病毒分别处于不同分支，其核苷酸和氨基酸分别存在3．7％和0％的差异。结论北京市2007年手足口病疫情主要由EV71和CoxA16两种病原引起，而每种病原都存在2个或2个以上流行株病毒；且CoxA16的传播似乎较EV71更为广泛。
Objective To identify the etiology of 8 human hand-foot-mouth disease （HFMD） outbreaks in Beijing, during May to July 2007. Methods Reverse transcription-polymerase chain reaction （RT-PCR） method was used to directly type the specimens including fluid from the herpes and throat swabs from the HFMD patients. Using RD cell lines, the collected stool specimens were cultured followed by typing. Partial VP1 region of selected EV positive specimens and cultures were sequenced and both nucleic acid sequence and predicted amino acid sequence were analyzed. Results The two HFMD outbreaks in Daxing region in Beijing in 2007 were caused by enterovirus 71 type （EV71）, and the others were caused by Coxsackie virus A16 （Cox A16）. Two EV71 strains caused epidemics in Daxing region in 2007 belonged to C4 subgenotype but on different branches in VP1 gene phylogenetic tree. The differences on nucleic acid sequence and amino acid sequence were 3.7% and 0.8% between the two EV71 stains, respectively. The Cox A16 strain in Shunyi region and the other strains were on different branches in phylogenetic tree, and the difference on nucleic acid and amino acid sequence were 3.7% and 0% respectively between the two Cox A16 stains. Conclusion The HFMD outbreaks occurred in Beijing in 2007 were caused mainly by EV71 and Cox A16, and there were two individual epidemic virus strains. Cox A16 seemed to spread more widely than EV71 in Beijing, 2007.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2009年第8期784-787,共4页
Chinese Journal of Epidemiology
