摘要
本实验采用改良纸片相邻法，以头孢西丁为诱导剂，头孢哌酮和头孢噻甲羧肟分别为靶β-内酰胺药物。检测1996年7月～1997年3月临床分离出的193株常见革兰阴性杆菌诱导型β-内酰胺酶的情况。发现有81菌株42％（81／193）出现诱导型β-内酰胺酶（即所谓的截平现象），其中能达到Sander判定为诱导株标准的有24株菌，占12．4％。临床常见革兰阴性杆菌中易产生诱导性肝内酸胶酶以阴沟肠杆菌、黄杆菌、铜绿假单胞菌多见。利用诱导剂一靶β-内酰胺药物的纸片相邻法与革兰阴性菌的日常药敏的有机结合，可使临床实验室在报告药敏结果同时予以报告产诱导酶的情况，以供临床用药时参考。
A modified disk approximation test, i. e. cefoxitin as an inducer in the center and two target beta-lactams cefoperazone and ceftazidirne placed bilaterally, was used to detect 193 Gram negative strains isolatedfrom Jun. 1996 to Mar. 1997 for inducible β-lactamase (IB) production. It was found that 81/193(41. 97% )strains showed IB positive with a truncate effect, among them, only 24 strains (12. 43 % ) were truly IBstrains by Sander's ctriteria. Pseudomonas, E. cloacae and Flavobacterium were the most comraon seen IBstrains in these Gram negative bacilli. Combination of inducer-targets approximation and routine susceptibility tests will provide clinical laboratory not only a on-time sensitivity report but as well as inducible β-lactamase production observation for Gram negative bacilli.
出处
《上海医学检验杂志》
1998年第2期94-95,共2页
Shanghai Journal of Medical Laboratory Sciences
