摘要
从临床患病牛乳样内分离出的金黄色葡萄球菌β-溶血型致病菌株,命名为zfb,运用微生物学及现代分子生物学手段检测了该菌株的生物学特性,以金葡菌ATCC25923为对照株。结果表明,金黄色葡萄球菌zfb不产生脂酶,有抗药性,在改良型血清软琼脂培养基中观察荚膜形态,活体内和体外均显现散布型状态。同时,以昆明小白鼠为动物模型,测定了半数致死量和器官侵袭力。金黄色葡萄球菌牛源分离株zfb的半数致死率为10-4.33/mL,参考菌株ATCC25923菌株的半数致死率为10-2.5/mL。对牛源金黄色葡萄球菌zfb的特性检测使其成为疫苗制备的候选者,为进一步研究其致病机理及制备减毒活疫苗奠定了基础。
A Staphylococcus aureus strain, designated zfb, was isolated from a clinical bovine mastitis case of a dairy cow. Staphylococcus aureus zfb can have resistance to methicillin and no lipase contrast by ATCC 25923. The production of the capsule was assessed by the diffuse colonial morphology in serumsoft agar. A mouse infection model was used to determine the LD50 and the invasiveness of SA zfb. The LD50 of SA 25923 to experimental mice was 10-25/mL, and the LD50 of SA zfb to experimental mice was 10M33/mL. The purpose to detect characteristics of SA zfb makes it an interesting candidate for the preparation and assay of an avirulent mutants against staphylococcal infections and further investigate on pathogenic mechanism.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第8期1206-1211,共6页
Microbiology China
基金
山东省自然科学基金(No.Y2007D10)
山东省农科院高技术创新基金(No.2006YCX028)
