摘要
背景:祖国医学中"肾主骨"的理论符合现代医学骨代谢调节机制,依此"阴中求阳法"和"阳中求阴法"理论研制的补肾复方在治疗骨质疏松症虽取得较好效果,但是滋补肾阴药与温补肾阳药配伍比例需要进行骨形成机制的深入探讨。目的:对比观察依照阴中求阳法组成的补肾方药中滋补肾阴药与温补肾阳药不同配伍比例对卵巢切除所致骨质疏松大鼠的治疗作用,以探讨它们之间的疗效是否存在差异。设计、时间及地点:随机对照动物实验,于2008-01/2009-01在河北医科大学中西医结合基础实验室完成。材料:清洁级3个月龄健康雌性SD大鼠(未曾交配)90只,只随机分成9组:正常对照组、假手术组、卵巢切除组、91组、82组、73组、64组、55组、补肾复方组,每组10只。补肾方药由地黄、淫羊藿、山药、丹参、骨碎补、独活等药物组成。方法:正常组和假手术组不造模,其余各组均切除卵巢造成骨质疏松症模型。91组、82组、73组、64组、55组分别按补肾阴药地黄∶补肾阳药淫羊藿为9∶1,8∶2,7∶3,6∶4及5∶5的比例配伍;生药含量均为960g/L,每天灌服7μL/g。补肾复方组按照地黄∶淫羊藿∶淮山药∶丹参∶骨碎补:独活等于8∶4∶4∶3∶3∶3的配伍比例制成的口服剂,生药含量约为1010g/L,每天灌服7μL/g,以上给药,1次/d,连续6d,休息1d后,再连续灌服6d,如此至3个月止;假手术组和正常对照组大鼠灌服等容积的蒸馏水。于卵巢切除1周后给药。主要观察指标:3个月后处死大鼠。普通光镜下观察骨小梁体积百分比、骨小梁吸收表面百分比及骨小梁形成表面百分比。荧光镜下观察活性生成表面百分比、骨小梁矿化率及骨小梁骨生成率。结果:90只大鼠均进入结果分析。切除卵巢3个月后,大鼠胫骨骨小梁体积百分比显著降低,骨小梁吸收表面百分比以及骨小梁形成表面百分比、活性生成表面百分比、骨小梁矿化率、骨小梁骨生成率皆显著增高。给药3个月后,与切除卵巢模型组比较,给大鼠灌服不同配伍比例滋阴药和温阳药组成的补肾方药3个月后,均能使上述指标发生逆转,但程度有所不同,补肾阴药地黄和补肾阳药淫羊藿配伍比例之中以7∶3比例为最明显。结论:根据"阴中求阳法"理论组成的补肾方剂,滋补肾阴药与温补肾阳药不同配伍比例对卵巢切除所致的骨质疏松均有明显的治疗作用,补肾阴药和补肾阳药配伍比例应该是"补阴药"大于"补阳药"疗效更好。
BACKGROUND： According to the theory of the kidney is in charge of the bones and the mechanism of bone metabolic regulation in modern medicine, the tonifying kidney prescriptions of reinforcing Yang from Yin and reinforcing Yin from Yang has effective action for osteoporosis, but further researches should focus on the action mechanism of the ratio of compatibility of the medicine for nourishing the Kidney-Yin and the medicine for warming and recuperating the Kidney-Yang at skeleton formation. OBJECTIVE： To compare the treatment effects of reinforcing Yang from Yin of the ratio of compatibility of the medicine for nourishing the Kidney-Yin and the medicine for warming and recuperating the Kidney-Yang on ovariectomy （OVX）-induced osteoporosis in rats. DESIGN, TIME AND SETTING： A randomized control animal experiment was carried out in the Basic Laboratory of Integrated Chinese Medicine and Western Medicine, Hebei Medical University between January 2008 and January 2009. MATERIALS： Ninety healthy female SD non-copulated rats of 3 months old and clean grade were randomly divided into nine groups： normal control group, sham operation group, OVX group, OVX plus 91 group, OVX plus 82 group, OVX plus 73 group, OVX plus 64 group, OVX plus 55 group and prescription of tonifying kidney group, with 10 rats in each group. The prescription for tonifying kidney comprised Rehmannia glutinosa, Epimedium grandiflorum, Dioscorea opposite, salviae miltiorrhizae, Drynaria fortunei and Angelica pubescens Maxim. METHODS： The osteoporosis model was induced in rats by the OVX with the exception of normal control group and sham operation group. The ratio of compatibility was 9：1, 8：2, 7：3, 6：4, 5：5 of Rehmannia glutinosa in prescription for nourishing the Kidney-Yin to Epimedium grandiflorum in prescription of warming and recuperating the Kidney-Yang, respectively. The crude drug content was 960 g/L, 7 μL/g gavage per day. The prescription of tonifying kidney consisted of Rehmannia glutinosa, Epimedium grandiflorum, Dioscorea opposite, salviae miltiorrhizae, Drynaria fortunei and Angelica pubescens Maxim at a ratio of 8：4：4：3：3：3. The crude drug content was 1 010 g/L, 7 μL/g gavage per day. The medication was commence from one week after operation and lasted continuously for 3 months with an interval of 1 day following 6 successive days. The rats of normal control group and sham operation group were given equal volume of distilled water from one week following OVX. MAIN OUTCOME MEASURES： All rats were killed at 3 months postoperation. The percentage of trabecular bone volume （TBV%）, the percentage of trabecular resorption surface （TRS%） and the percentage of trabecular formation surface （TFS%） were observed under light microscopy; the percentage of active formation surface （AFS%）, mineral apposition rate （MAR） and bone formation rate （BFR） were observed under fluoroscope. RESULTS： All 90 rats were involved in the result analysis. Three months after OVX, the TBV% in tibia reduced markedly, but TRS%, TFS%, AFS% , MAR , BFR heightened markedly. Compared with the OVX model group, the prescriptions for nourishing the Kidney-Yin or warming and recuperating the Kidney-Yang at the different ratios of compatibility for three months could changed these indices to some extents. The potent effect of tonifying kidney treatment was the ratio of nourishing the Kidney-Yin （Rehmannia glutinosa） 7 to warming and recuperating the Kidney-Yang Epimedium grandiflorum） 3. CONCLUSION： According to the tonifying kidney prescription based on the theory of reinforcing Yang from Yin, both the rescription for nourishing the Kidney-Yin and warming and recuperating the Kidney-Yang have effects on treating the OVX-induced osteoporosis in rats, and the effect of the former is better than the latter.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第33期6478-6482,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30472200)
河北省引进留学人员经费资助项目(200828)~~
