摘要
目的；探讨用酶联免疫吸附法（ＥＬＩＳＡ）测定抗ＥＢ病毒早期抗原ＩｇＧ抗体水平（ＩｇＧ／ＥＡ）在鼻咽癌血清学诊断中的应用。材料和方法：用ＥＬＩＳＡ方法和免疫酶染色方法分别检测５２４例健康人和１２１例鼻咽癌病人血清的ＩｇＧ／ＥＡ水平（ＯＤ）和血清ＩｇＡ抗体对ＥＢ病毒壳抗原（ＩｇＡ／ＶＣＡ）的滴度。结果：以ＯＤ≥０１８作为ＥＬＩＳＡ的阳性值，鼻咽癌诊断的特异性和敏感性分别为９４％（４９３／５２４）和９０％（１０９／１２１），与ＩｇＡ／ＶＣＡ阳性滴度≥１∶８０为标准获得的鼻咽癌诊断特异性９６％（５０３／５２４）和敏感性８９％（１０８／１２１）相一致。以“双阳性”（ＩｇＧ／ＥＡＯＤ≥０１８，ＩｇＡ／ＶＣＡ滴度≥１∶８０）作为鼻咽癌标准，诊断的敏感性为７７％（９３／１２１），特异性１００％（５２４／５２４）。上述结果在１３４例鼻咽癌高危血清样品的测定中得到重复。结论：（１）用ＥＬＩＳＡ测定血清ＩｇＧ／ＥＡ抗体水平也适用于鼻咽癌人群普查。（２）为了提高鼻咽癌血清学诊断的特异性，建议采用“双阳性”（如ＩｇＧ／ＥＡＯＤ≥０１８，ＩｇＡ／ＶＣＡ滴度≥１∶８０）作为鼻咽癌人群普查中的血清学阳性标准。
Abstract Purpose: To study the significance of detection for IgG antibody to EB virus early antigen (IgG/EA) by enzyme linked immunosorbent assay (ELISA) in NPC serologic diagnosis. Materials and Methods: Sera samples from 524 cases of health persons and 121 cases of NPC were tested by ELISA and immunoenzymatic staining Method (IESM) for the levels (OD) of IgG/EA and titers of IgA antitody to EBvirus capsid antigen (IgA/VCA). Results: Using OD≥018 as ELISA positive cut off value, the diagnostic specificity and sensitivity to NPC were 94% (493/524) and 90% (109/121) respectinely. These were the same as those tested on IgA/VCA(+) titer ≥ 1∶80, 96%(503/524) and 89% (108/121) respectively. According to 'dual positive' (IgG/EA OD ≥ 0.18, IgA/VCA titer ≥ 1∶80)as NPC criteria, the diagnostic sensitivity of 77% (93/121) and specificity of 100%(524/524) were obtained. Same results were repeated in the detection of 134 Samples from highrisk population of NPC. Conclusion: (1) The detection of IgG/EA by ELISA was also adaptable in mass survey for NPC. (2) In order to elevated serologic diagnostic specificity to NPC, it is worth to take 'dual positive' (e.g. IgG/EA OD ≥0.18 and IgA/VCA titer ≥ 1∶80) criteria as NPC threshold in mass survey.Subject word EBvirus Early antigen Nasopharyngeal Neoplasms ELISA
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1998年第4期281-282,285,共3页
Chinese Journal of Cancer
基金
广东省卫生厅科研基金
