摘要
目的检测表皮葡萄球菌hld基因在抗菌药物MIC浓度作用下在生物膜形成、细菌脱离过程中的转录水平变化,探索抗菌药物是否通过影响hld基因转录,介导了生物膜相关的耐药和持续感染。方法以MIC浓度4种抗菌药物作用于表皮葡萄球菌,采用SYBR实时荧光定量RT-PCR方法检测表皮葡萄球菌生物膜形成解离不同时段hld基因的转录水平变化,并与无药对照组相应时段的转录水平相对照,了解抗菌药物对hld基因转录的影响。结果各组细菌hld基因转录水平从黏附时开始下降,随后数小时持续下降,对照组在24 h后缓慢上升,72 h与2 h比较差异有统计学意义,而抗菌药物组无明显上升,与对照组比较差异有统计学意义。结论抗菌药物通过对hld基因转录水平的影响,使表皮葡萄球菌黏附作用增强,后期可防止输水通道基质解离和细菌脱离,降低药物渗入,从而导致抗菌药物耐药。
OBJECTIVE To investigate the transcription level of gene hld of Staphylococcus epidermidis in the biofilm forming and detaching under MIC antibiotic and explore the relationship between biofilm-related drug resistance and persistant infection. METHODS The transcription level of gene hld of S. epiderrnidis under MIC concentration of 4 antibiotics was compared with those of the control group without antibiotics by SYBR real-time fluorescent quantitative RT-PCR at the different time point of biofilm formation and detachment. RESULTS The transcription of gene hld decreased rapidly from initial adherence, and droped continuously for few hours. There was an increase from 24 hours to 72 hours in groups without antibiotics but not in antibiotics groups, the differenet was significant. CONCLUSIONS Antibiotics improve adherence at first and then prevent matrix decomposition water-conducting tube and detachment of cells by impact of transcription of gene hld, it can protect cells from killing by inhibiting the penetration of bioties and prevent them become planktonic cells after detachment from biofilm.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第16期2071-2074,共4页
Chinese Journal of Nosocomiology
关键词
表皮葡萄球菌
生物膜
耐药
hld基因
转录
Staphylococcus epiderrnidis
Biofilm
Drug resistance
Gene hld
Transcripition
