摘要
为了探讨抑癌蛋白对胃癌细胞增殖和凋亡的影响，以阐明胃癌恶性生长的可能机理，选择８８例手术切除的胃溃疡和胃癌的蜡块标本，将ＢｒｄｕＴＰ掺入到ＤＮＡ的断端，结合抗Ｂｒｄｕ抗体放大信号，再用免疫组化的方法检测组织中的凋亡细胞。同时采用免疫组化方法检测这些组织中的ｐ２１、ｐ５３及ＰＣＮＡ的表达。结果显示，胃癌组织中的细胞增殖水平高而凋亡细胞减少。癌巢中很少有凋亡细胞。胃溃疡组织中凋亡的细胞明显多于胃癌组织。ｐ５３蛋白在胃癌中的检出率为３８．０％。ｐ２１蛋白在胃粘液腺癌及低分化、未分化型腺癌中检出率很低。结果表明，尽管胃癌组织中发现抑癌基因ｐ５３突变蛋白的表达水平增高，但并不能促使胃癌细胞的增殖，却可以增加癌细胞的凋亡。胃粘液腺癌和低分化、未分化型腺癌的抑癌蛋白ｐ２１的表达水平低下可能是该类癌细胞过度增生的原因之一。
The effect of tumor supressor protein on the cellular proliferation and apoptosis was studied for understanding the possible mechanism of malignant growth of stomach cancer.88 paraffinembeded samples of gastric ulcer and stomach cancer from surgical resected tissues were selected.Apoptosis in these samples was detected by terminal labeling method.The expression of p53 and p21 protein were detected by immunohistochemical method.The results showed that the cellular prolifirative level was increased and the apoptotic cells was decreased in stomach cancer tissues.There were rarely apoptotic cells in nestedcancer.The opposite results were found in the tissues of gastric ulcer.Positive rate of p53 protein in stomach cancer sample was 40%.There was low positive rate of p21 protein in the low and undifferentiation type of adenocarcinoma and mucinous adenocarcinoma of stomach.The results suggest that the cells in the stomach cancer tissues grow rapidly and apoptosis is inhibited.Although the expression of p53 mutated protein and p21 protein is abnormal,no relation of the cellular proliferation and apoptosis with the expression of p53 and p21 protein are found.
出处
《实用癌症杂志》
1998年第3期193-195,共3页
The Practical Journal of Cancer
