摘要
目的验证类胰岛素生长因子结合蛋白-6(IGFBP-6)是否可以作为增生性玻璃体视网膜病变(PVR)的血清分子标志物。方法收集PVRA、B、C、D级玻璃体(n=8)及术前术后6个月血清样本(n=20),PVR C级以上为严重PVR。正常供体眼玻璃体样本(n=8)和健康体检者血清(n=20)作为正常对照,对玻璃体和相应的血清样本分别进行IGFBP-6的免疫印迹分析和酶联免疫吸附试验(ELISA)。环扎术后未愈者(n=8)和硅油眼(n=8)患者的血清样本进行ELISA分析。在临床研究中的个体使用方面符合赫尔辛基宣言,参与试验者签署知情同意书。结果22例PVR患者(总体24例)玻璃体和血清中可检测到IGFBP-6,而供体眼玻璃体和正常人的血清中未测到。PVRC级、D级中IGFBP-6条带明显强于PVR B级。ELISA结果显示严重PVR患者玻璃体和血清中IGFBP-6质量浓度明显高于轻度PVR(F=3.34,P=0.04)。PVR患者术后6个月血清中IGFBP-6由(185.3±34.9)pg/mL明显下降至(65.4±31.8)pg/mL(t=11.10,P=0.015),与玻璃体术后硅油眼和正常对照组的血清中的质量浓度接近(t=0.08,P=0.989;t=1.59,P=0.131),但明显低于环扎术后未愈组(t=3.16,P=0.009)。结论IGFBP-6是PVR患者玻璃体和血清的特异蛋白质,与PVR的严重程度和预后评估相关,可作为PVR血清分子标志物。
Objective This study was to determine whether insulin-like growth factor binding protein 6 （IGFBP-6） is a serum biomarker of the proliferative vitreoretinopathy （PVR）. Methods The vitreous samples （n = 8 ） were collected from the eyes with grade A,B, C, D of PVR respectively during intraoperation, and serum samples （n = 20） were obtained preoperatively and 6 month postoperatively. The vitreous samples of donor eyes （n = 8） and serum samples of normal population age- and gender-matched （n = 20） were as normal controls. Western blotting analysis and enzyme linked immunosorbent assay （ELISA） were used for the detection of IGFBP-6 in vitreous and serum. The serum samples of encircling failure group （ n = 8 ） and silicone group （ n = 8 ） were analyzed by ELISA. Trial procedure followed the Statement of Helsinki. Written informed consent was obtained at initial of any clinical trial. Results Western blotting analysis displayed that IGFBP-6 was detected in the vitreous and serum samples in 22 PVR patients out of 24 patients rather than the donor vitreous and normal serum samples. The band intensity of IGFBP-6 was higher in the samples from grade C and D of PVR than that of grade B. The concentration of IGFBP-6 in vitreous was significantly different among different grades of PVR groups（ F = 3.34, P = 0. 04）. The ELISA outcomes documented that IGFBP-6 concentrations in vitreous and serum samples were significantly higher in severe PVR patients than those in moderate PVR. At 6 months after postoperation,IGFBP-6 level in serum of PVR was significantly lower than that of preoperation [ （65. 4 ±31.8）pg/mL vs （ 185. 3±34. 9）pg/mL（t = 11.10,P =0. 015） ] and encircling failure group（ t = 3. 16 ,P = 0. 009 ）, but was similar to that of silicone group and normal group （ t = 0. 08, P = 0. 989 ; t = 1.59, P = 0. 131）. Conclusion IGFBP-6 is a special protein in vitreous and serum of the PVR patients. The concentration of IGFBP-6 is correlated with the severity and prognosis of PVR. Therefore,IGFBP-6 can be the candicated serum hiomarker of PVR.
出处
《眼科研究》
CAS
CSCD
北大核心
2009年第8期641-644,共4页
Chinese Ophthalmic Research
基金
国家自然科学基金(30870558)
上海市启明星计划(09QA1405100)
上海市卫生局基金(2008179)
同济大学优秀青年人才培养计划(2008KJ068)资助
