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高糖及高胰岛素对肺泡巨噬细胞氧化应激的影响 被引量：6

Effect of high glucose or high glucose and high insulin on oxidative stress of activated rat alveolar macrophages
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摘要 目的:探讨高糖、高糖+高胰岛素环境对大鼠肺泡巨噬细胞(AM)氧化应激损伤的影响。方法:采用支气管肺泡灌洗的方法分离大鼠AM,高糖及高糖+高胰岛素环境下培养,用卡介苗(BCG)、干扰素α-2b(IFNα-2b)或两者联合活化,检测其丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和乳酸脱氢酶(LDH)活性。结果:BCG和IFNα-2b+BCG活化AM时,其SOD活性均低于对照组(P<0.05),而MDA含量和LDH活性均高于对照组(P<0.05);IFNα-2b活化AM时,其MDA含量、LDH活性和SOD活性与对照组无明显差别(P>0.05)。结论:在高糖、高糖+高胰岛素环境可诱导AM氧化应激损伤,从而使糖尿病个体易发生肺部感染。
作者 沈兴平 舒昌达 何军
机构地区 厦门大学附属中山医院内分泌科 重庆医科大学附属第一医院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2009年第8期1650-1651,1659,共3页 Chinese Journal of Pathophysiology
基金 福建省自然科学基金资助项目(No.X0750055)
关键词 巨噬细胞 肺泡 氧化性应激 高糖 高胰岛素 Macrophages,alveolar Oxidative stress High concentration of glucose High concentration of insulin
分类号 R587.1 [医药卫生—内分泌]
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参考文献6

二级参考文献16

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共引文献82

同被引文献74

  • 1郝新忠,陈隽,杨妤欣,刘云,郑瑞芝.2型糖尿病大鼠活化肺泡巨噬细胞CD14表达及TNF-α、IL-6分泌减少[J].中国糖尿病杂志,2006,14(1):52-55. 被引量：5
  • 2迟梅英,潘晓军,赵勇,张雷,李风光,张彦伟.异丙酚对高氧肺损伤大鼠氧自由基、IL-8及肺组织超微结构的影响[J].齐鲁医学杂志,2007,22(2):122-125. 被引量：5
  • 3汤秀英,王昱,王书合,张烨,柴立军,郭晓惠.糖尿病大鼠肺的超微结构观察及形态计量学研究[J].中国体视学与图像分析,2007,12(2):126-128. 被引量：11
  • 4盖红波,胡英红,修学宝,赵树燕.糖尿病下呼吸道医院感染临床特点分析[J].中华医院感染学杂志,2007,17(7):801-802. 被引量：26
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引证文献6

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中国病理生理杂志
2009年 第8期

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