摘要
目的研究蒙古黄芪凝集素(AMML)对慢性髓系白血病细胞系K562的生长抑制作用及对细胞周期和凋亡的影响。方法用MTT法测定AMML对K562细胞的生长抑制作用;荧光染色和流式细胞术分析检测AMML诱导K562细胞周期变化和凋亡的影响。结果AMML对K562细胞的生长增殖具有明显的抑制作用,呈剂量和时间依赖性。60mg.L-1的AMML处理K562细胞72h后其生长抑制率高达89%。AMML处理的K562细胞呈现典型的细胞凋亡形态改变,如胞核破裂、染色质固缩。流式细胞仪(FCM)分析结果说明AMML可以引起K562细胞S期阻滞,并诱导K562细胞凋亡。结论AMML通过S期阻滞抑制K562细胞的增殖并诱导其凋亡,AMML在抗肿瘤新药开发中具有潜在的应用价值。
Aim To investigate the effects of Astragalus Mongholicus Bunge Lectin （AMML） on tumor cells proliferation, cell cycle and apoptosis by using human leukemia cell line （ K562 cells）. Methods The anti-proliferation effect of AMML on K562 cells was detected by the colorimetric MTT assay. The apoptosis induced by AMML on K562 cells was explored by means of cell morphological and flow cytometry. Results AMML showed strong inhibiton of the growth of K562 cells in a time-and concentration-dependence. After incubation of K562 cells with AMML at a concentration of 60 mg·L^-1 for 72 h, the inhibition ratio was 89%. Morphological observation showed that AMML-treated K562 cells displayed outstanding apoptosis characteristics, such as nuclear fragmentation, chromatin condensation. AMML induced significant cell cycle arrest at S phase in K562 cells, and the apoptosis of K562 cells was confirmed by flow cytometry. Conclusion AMML can inhibit the growth of K562 cells through S arrest and induce the apoptosis of K562 cells. Thus, AMML may be valuable for the treatment of cancer.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第7期904-907,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No20502032)
教育部"新世纪优秀人才支持计划"资助项目(NoNCET-08-0534)
