摘要
选取长势较好、无病害野生白花败酱植株的茎尖及茎段作为外植体进行组织培养,以MS为基本培养基,设计不同激素浓度配比试验,筛选最佳培养基配方。结果表明:采用0.1%HgCl2对外植体进行消毒,茎尖最佳消毒时间为10 min,茎段为15 min;最佳诱导培养基配方是MS+6-BA 3.0 mg/L+NAA 0.1 mg/L,诱导率100%,增殖培养基MS+6-BA 2.0 mg/L+NAA0.2 mg/L,增殖倍数7.8;生根壮苗培养基是MS+NAA 1 mg/L+马铃薯5%。
Took the shoot tips of well-grown, virus-free wild Patrinia villasa Juss as explants, the micropropagation system of Patrinia villasa Juss was established. The research showed that, the prefer able sterilized protocol was to take 15 rain of shoot length as explants, and surface sterilized with 0. 1% HgCl2 solution for 10 rain. The most effective induction and subculture media were MS basal medium supplemented with 3. 0 mg/L BA and 0. 1 mg/L NAA which got 100% induction frequency, with 2.0 mg/L BA and 0. 2 mg/L NAA which got 7. 8 times of proliferation separately. The better rooting medium was MS basal medium supplemented with 1.0 mg/L NAA and 5% （w/v） mashed potato.
出处
《北方园艺》
CAS
北大核心
2009年第8期121-123,共3页
Northern Horticulture
关键词
白花败酱
茎尖
茎段
诱导
生根
Patrinia villasa Juss
Stem tip and shoot
Induction
Rooting
