摘要
对设施葡萄衰老时叶片中叶绿素含量、可溶性蛋白质、膜的透性、丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化物酶(POD)进行了测定和理论分析,以研究不同上市时期的设施红地球栽培后期叶片衰老时的生理变化特征。结果表明:促早和延后栽培条件下葡萄衰老时叶片的叶绿素含量都在缓慢减少,延后栽培叶绿素含量显著高于其它处理;可溶性蛋白质含量呈现先升后降的趋势,其中露地环境下叶片内蛋白质含量的最大值比其他处理的最大值高但后期下降较快,延后栽培的叶片可溶性蛋白含量较低但后期下降较缓慢;葡萄叶片细胞膜的相对透性在生育后期都呈上升趋势,露地(CK)>促早2>延后≈促早1;促早和延迟栽培条件下葡萄衰老时叶片MDA呈普遍上升趋势,在葡萄生育后期(9月16日以后)MDA含量表现为露地>促早1>促早2>延后;促早和延后栽培条件下葡萄后期叶片SOD酶的活性呈双峰曲线,都表现为延迟>露地>促早,而POD活性的变化都呈现先升高再下降的趋势,延后栽培的POD活性显著高于促早和露地葡萄;葡萄叶片衰老过程中叶绿素、蛋白质、SOD和POD活性在9月25日以后都表现出相同的缓慢下降趋势,而膜透性和MDA含量明显上升,说明从这个时候起植物正式进入衰老期。
In order to study the physiological changes of Red Global grape leaves in their early or postponed cultivation, chlorophyll,protein,POD, SOD, MDA and membrane permeability were tested. The result showed： All chlorophyll content went down gently, but chl. of the postponed cultivation was above others obviously; The protein of all went up first, then went down, the CK＇s curve was above others, but went down quickly in the end; the postponed cultivation curve was the lowest, but goes down gently in the end; All membrane permeability went up totally, CK〉early cultivation 2〉postponed cultivation ≈early cultivation 1; All MDA went up totally, after Sept. 16, CK〉early cultivation 1〉 fearly cultivation 2〉postponed cuhivation; Tbe SOD of all showed two peaks curve, postponed cultivation 〉CK〉 early cultivation; the curve of POD first went up then goes down, and the POD of postponed cultivation was above others remarkably; After Sept. ZS, chl, protein, SOD activity and POD activity all went down gently, while membrane permeability and MDA went up remarkably, so we could say the plant begin to senesce from this time.
出处
《北方园艺》
CAS
北大核心
2009年第8期40-44,共5页
Northern Horticulture
基金
国家科技支撑计划资助项目(2007BAD57B05)
关键词
葡萄叶片
衰老
生理变化
Grape leaves
Senescence
Physiological changes
