摘要
以豆粕水解液为原料,研究根霉产纤溶酶液体发酵培养基的组成。实验结果表明,豆粕的最适水解时间为1.5 h,麸皮水与豆粕水解液的最适体积比例为2∶3,金属离子对酶活力提高无显著作用,最适培养基配方是:5%麸皮水,6%豆粕水解液,0.1%氯化铵,0.2%硝酸钠,2.5%胰蛋白胨,初始pH4.3,酶活力为177.62 U/mL。
The components of liquid fermentation medium of fibrinolytic enzyme produced by Rhizopus chinesis were studied,in which soybean cake hydrolysate was used as substrate. The optimal conditions were obtained as followed ： hydrolysis time of soybean cake 1.5 h, the ratio of wheat bran water and soybean cake hydrolysate 2：3. The metal ions had no significant effects on enzyme activity. The optimal medium composition was as followed： wheat bran water 5% ,soybean cake hydrolysate 6% ,NH4Cl 0. 1% ,NaNO3 0. 2% ,tryptone 2. 5% ,initial pH 4. 3. Under these conditions, the activity reached 177. 62 U/ml.
出处
《中国酿造》
CAS
北大核心
2009年第8期60-62,共3页
China Brewing
基金
黑龙江省自然科学基金资助项目(D0228)
关键词
根霉
纤溶酶
液体发酵
Rhizopus chinesis
fibrinolytic enzyme
liquid fermentation
