摘要
目的观察Ⅰ、Ⅲ型前胶原ｍＲＮＡ和胶原蛋白及硫酸软骨素蛋白聚糖及其基因在正常和有动脉粥样硬化病变的人冠状动脉内的表达和分布，及Ⅰ、Ⅲ型胶原增生和硫酸软骨素蛋白聚糖增多在人冠状动脉粥样硬化病变形成过程中的作用。方法应用ＨＥ、天狼星红和免疫组化染色比较人冠状动脉不同类型病变及早期斑块和晚期斑块病变的Ⅰ、Ⅲ型胶原和硫酸软骨素蛋白聚糖的分布；应用原位核酸分子杂交技术，比较分析正常及斑块部位的Ⅰ、Ⅲ型前胶原ｍＲＮＡ，ｂｉｇｌｙｃａｎ及ｄｅｃｏｒｉｎｍＲＮＡ的表达。结果（１）随病变的进展，冠状动脉内膜中Ⅰ型和Ⅲ型胶原和硫酸软骨素蛋白聚糖的分布增多，斑块部位Ⅰ型和Ⅲ型前胶原ｍＲＮＡ和ｂｉｇｌｙｃａｎ及ｄｅｃｏｒｉｎｍＲＮＡ表达增高；（２）硫酸软骨素蛋白聚糖与β脂蛋白在斑块病变中的分布有相似的定位；（３）早期斑块内平滑肌细胞增殖较活跃，以Ⅲ型胶原增多为主，硫酸软骨素蛋白聚糖增多显著。晚期斑块内平滑肌细胞数量减少，多以Ｉ型胶原增多为主，硫酸软骨素蛋白聚糖分布减少。结论动脉粥样硬化病变时Ⅰ、Ⅲ型前胶原ｍＲＮＡ和ｂｉｇｌｙｃａｎ及ｄｅｃｏｒｉｎｍＲＮＡ的过度表达与Ⅰ型、Ⅲ型胶原和硫酸软骨素蛋白聚糖的增多相关；动脉壁内平滑肌细胞?
Objective To investigate the expression of type Ⅰ、Ⅲ procollagen mRNAs and chondroitin sulfate proteoglycan (CSPG) genes, as well as the distribution of type Ⅰ、Ⅲ collagen fibers and CSPG in human coronary atherogenesis. Methods Staining for HE, picrosirius red, immunohistochemisty and hybridization in situ technique were included. Results (1) Along with the development of athrosclerotic lesion, collagen type Ⅰ、Ⅲ and CSPG were increased simultaneously in the coronary wall; The expressions of type Ⅰ、Ⅲ procollagen, biglycan, and decorin mRNAs were noticed to be increased in the plaques. (2) β lipoprotein was seen coexistently with CSPG in the AS plaques of the coronary arteries. (3) There were more SMC proliferation, predominant augmentation of type Ⅲ collagen fibers and accumulation of CSPG in the early plaques of most young subjects; but less SMC, amount of type I collagen fibers and deposition of CSPG in the advanced plaques of most aged people. Conclusion Over expression of type Ⅰ、Ⅲ procollagen, biglycan and decorin mRNAs was related to the increase of type Ⅰ、Ⅲ collagen and CSPG in the atherosclerotic lesions. Proliferation of SMC, augmentation of type Ⅰ、Ⅲ collagen and CSPG and change of the proportion between collagen and CSPG are considered to play an important role in the development of coronary atherosclerosis.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1998年第3期177-184,共8页
Chinese Journal of Pathology
基金
国家"八五"科技攻关课题
关键词
胶原
蛋白硫酸软骨素
冠心病
细胞外基质
Coronary arteriosclerosis Collagen Proteochondroitin sulfates Immunohisto chemistry In situ hybridization
