摘要
目的：ELISA方法检测HBV感染标志物，观察不同血清学指标组合的HBV-DNA阳性情况。方法：回顾分析2006-2008年我院就诊乙肝患者778例，用荧光定量聚合酶链反应和酶联免疫吸附试验（ELISA）分别检测其血清的HBV-DNA和HBV血清标志物（HBV-M）。结果：1组[（HBsAg、HBeAg、抗HBc阳性）、2组（HBsAg、抗HBc、抗HBc阳性）、3组（HBsAg阳性）的患者HBV-DNA的阳性率分别为96．7％、54．2％、18．8％，其拷贝数分别为1．62×10^8／ml，1．12×10^6/ml，7．61×10^4／ml。结论：荧光定量PCR对乙型肝炎早期诊断、传染性的判断及疗效观察具有临床实用及指导意义。
Objective：To investigate the positive HBV-DNA of different serological markers combination in patients with HBV infection. Methods：HBV-DNA and HBV serum markers in 778 patients with hepatitis B virus were detected by fluorescent quantitative PCR and ELISA from 2006 to 2008. Results：The positive rate of Groupl （HBsAg＋, HBeAg＋ and HBcAb＋） was 96.7%.The positive rate of Group2 （HBsAg＋,HbeAb＋ and HBcAb＋） was 54.2% and the positive rate of Group3（HBsAg＋）was 18.8%.Their copy counts were 1.62×10^8/ml, 1.12×10^6/ml and 7.61×10^4/ml respectively. Conclusions：Fluorescent quantitative PCR has applicative and guidance significance in early diagnosis and infectivity judgment and therapeutic effect assess.
出处
《中国医药导刊》
2009年第7期1200-1201,共2页
Chinese Journal of Medicinal Guide
