摘要
目的探讨银杏叶提取物（ＥＧｂ）的抗自由基保护心血管功能及其舒张离体脑血管的作用与释放ＮＯ的关系。方法（１）家兔心脏与离体主动脉环用二苯三硝基苯肼（ＤＰＰＨ）自由基造成心收缩功能障碍和乙酰胆碱诱发的血管舒张功能损伤与经ＥＧｂ预处理后再用ＤＰＰＨ损伤组比较。（２）观察ＥＧｂ对猪离体基底动脉环的舒张作用及其对电刺激诱发的舒张作用的影响。结果（１）ＥＧｂ能保护心脏与血管抗ＤＰＰＨ自由基对心收缩功能与血管内皮细胞舒张功能的损伤；（２）ＥＧｂ对猪基底动脉有量效依赖性的舒张作用，舒张内皮细胞健全的基底动脉的作用比除去内皮细胞的基底动脉更显著。（３）ＥＧｂ能增强电刺激基底动脉诱发的舒张作用，此作用可被Ｎ硝基精氨酸或河豚素毒所阻断。结论ＥＧｂ能保护家兔离体心脏与血管内皮细胞抗ＤＰＰＨ自由基损伤。对离体基体动脉有舒张作用，并能增强电刺激诱发的舒张作用，此作用与其促进ＮＯ释放有关。
Objective To examine the antifree radical related cardiovascular protective effects and NOmediated cerebrovasorelaxant effects of extract of the leaves of Ginkgo biloba(EGb) in isolated preparations. Methods Experiments on the antifree radical related cardiovascular protective effects were performed in rabbit Langendorff heart and isolated aortic rings damaged by diphenylpicryl hydyazyl(DPPH). Protective effects of EGb were determined by comparing the results of EGb pretreated group with the DPPHinjured controls.Cerebrovasorelaxant effects of EGb were examined in ring preparations of isolated porcine basilar artery in vitro using tissue bath techniques. Results EGb protected the isolated rabbit heart from the DPPHinjury to the cardiac contractility and the aortic endothelium from DPPHattenuated AChinduced relaxation. EGb concentrationde dependently relaxed the basilar artery and this effect was more significant in endothelium intact rings than those endothelium denuded rings. EGb enhanced the TNSinduced relaxation in basilar artery and this effect was abolished by pretreatment of NnitroLarginine or tetrodotoxin. Conclusions EGb exerts cardiovascular protection against DPPHimpaired cardiac contraction in rabbit isolated heart and endotheliumdependent relaxation in the aortic ring of rabbit in vitro. EGb relaxes porcine basilar artery and enhances the TNSinduced relaxation via a NO pathway.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第9期692-695,共4页
National Medical Journal of China
关键词
银杏叶提取物
心血管
保护作用
脑供血不足
Ginkgo biloba Free radical Cardiovascular system Porcine basilar artery Nitric oxide
