摘要
采用响应面法对植物乳杆菌的发酵产酶条件进行了优化。选取发酵时间、发酵温度、接种量和底物添加量作为考察因素,在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Benhnken)试验设计原理,采用4因素3水平的响应面分析法并依据回归分析确定了各工艺条件的影响因子,同时以亚油酸转化率为响应值作响应面和等高线,分析了各个因素的显著性和交互作用。植物乳杆菌产亚油酸异构酶的最佳发酵条件为:发酵时间为30.21h、发酵温度为36.23℃、接种量为1.99%、底物添加量为9.62%。在最佳发酵条件下,实际亚油酸转化率为20.46%。
Response surface methodology （RSM） was applied to evaluate the optimum fermentation conditions of Lactobacillus plantarum. Fermentation temperature, fermentation time, inoculation amount, and substrate concentration were chosen as the influencing factors by the single-factor experiments. The optimum conditions were obtained through Box-Behnken center-united experiment design and response surface methodology with 4 factors and 3 levels. Then the factors influencing the technological parameters were determined by regression analysis. Response surface and contour were finally graphed with the Linoleate isomerase （LA） conversion as the response value. The optimum fermentation conditions for the production of linoleate isomerase from L. plantarum were obtained as incubation time 30.21 hours, incubation temperature 36.23 degrees C, inoculation amount （volume ratio） 1.99 percent, and substrate concentration （volume ratio） 9.62 percent. The LA conversion was 20.46 percent under the optimum conditions.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2009年第7期106-108,共3页
Journal of Northeast Forestry University
关键词
植物乳杆菌
亚油酸异构酶
响应面分析
Lactobacillus plantarum
Linoleate isomerase
Response surface methodology
